Friday, November 21, 2003 Description Degradation of nuclear DNA can be examined by the TUNEL assay, which allows for in situ detection of nicked DNA and DNA breaks at the single cell level. This method is based on the enzymatic labeling w...

Friday, November 21, 2003 Description Protocol for Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction Procedure 1. Grind 2 to 5 g of frozen leaves to a very fine powder with a liquid nitrogen-cooled mortar and pestle. 2. Add 25 ml...

Friday, November 21, 2003 Description TMV is a helical rod with two components, an RNA of 6.1 kb and a protein of 17.5 kDa. Ninety-five percent of the mass is protein and 5% of the mass is RNA. To isolate the RNA you dissociate the two com...

Friday, November 21, 2003 Description This protocol describes how to determine the chlorophyll content of chloroplasts using a spectrophotometric assay. Procedure 1. Isolate Chloroplasts from spinach leaves (See protocol for preparation of...

Thursday, November 20, 2003 Description Please see the procedure Procedure 1. Flame one side of the slide in the flame of your Bunsen burner to remove any oil or grease. Allow the slide to cool. 2. Place a drop of water in the center of th...

Wednesday, November 19, 2003 Description No description Procedure Host bacteria (E. coli LE392)preparation: Pick one colony from fresh overnight culture on NZY agar plate and inoculate 50 ml NZY broth supplemented with 0.2% maltose and 10...

Wednesday, November 19, 2003 Description IMPORTANT TIPS: DO NOT at any time touch the gel with your fingers...gloved or ungloved...Any contact with the gel with result in a dark stain where the gel was touched. DO NOT use the water from an...

Tuesday, November 18, 2003 Description Rhodamine-Phalloidin/Calcofluor Staining Procedure Grow 50 mls of yeast to 5x10E6. Add formadehyde to the media to 4% (33 mls. of 10%). Fix in media at temperature for 10 min. Spin down cells 2-3 K fo...

Tuesday, November 18, 2003 Description Rhodamine-Phalloidin/Calcofluor Staining Procedure Grow 50 mls of yeast to 5x10E6. Add formadehyde to the media to 4% (33 mls. of 10%). Fix in media at temperature for 10 min. Spin down cells 2-3 K fo...

Tuesday, November 18, 2003 Description Yeast Cell Cycle by Flow Cytometry Procedure Spin down 107 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store...