Grants/prizes
1996: Grant 805-26.183 of the Netherlands Organization of Scientific Research (NWO/Stichting Levenswetenschappen): Molecular mechanisms of neuronal membrane traffic in synaptic vesicle recycling.
1998: UMCU-grant: Attraction and maintenance of female talent.
2000: Grant 8PJ/00-31 of the Netherlands Organization of Scientific Research (NWO/ALW): Identification and characterization of vesicular transport intermediates in axons.
2000: FOM Grant FBI-IIEOI/00-11: Electron tomography and 3D-analysis of intracellular compartments: The early biosynthetic pathway. With Dr. B. Koster (UU)
2001: Genentech, Inc., San Francisco, USA. Purchase order No. 559963. Project: Intracellular trafficking of Tumor Antigens and their Binding Antibodies.
2002: ISAO Award (International Foundation Alzheimer Research): Subcellular localization of the active g-secretase complex. Border crossing research grant with Prof. W. Annaert, University of Leuven.
2003: ZonMW investment grant: Advanced Live-cell Imaging station for multipurpose biomedical imaging.
2004: Intra-European Marie-Curie Fellowship (Motility 5): Defining the transport pathway of the receptor-mediated sorting of lysosomal enzymes by correlative live-cell imaging and electron-microscopy.
2004: ZonMW VICI award: Defining novel pathways of lysosome biogenesis: a genome-wide screen combined with correlative microscopy.
2005: Horizon Breakthrough grant. A semi-automated fluorescence microscopy-based RNA-interference screen to define novel pathways of lysosome biogenesis.
2005: internationaliserings subsidie

List of publications

1. Tager, J.M., R.P. Oude Elferink, A. Reuser, M. Kroos, L.A. Ginsel, J.A. Fransen, and J. Klumperman. 1987. Alpha-glucosidase deficiency (Pompe’s disease). [Review]. Enzyme 38:280-285.

2. Fransen, J.A., L.A. Ginsel, P.H. Cambier, J. Klumperman, R.P. Oude Elferink, and J.M. Tager. 1988. Immunocytochemical demonstration of the lysosomal enzyme alpha-glucosidase in the brush border of human intestinal epithelial cells. Eur. J. Cell Biol. 47:72-80.

3. Eilers, U., J. Klumperman, and H.P. Hauri. 1989. Nocodazole, a microtubule-active drug, interferes with apical protein delivery in cultured intestinal epithelial cells (Caco-2). J. Cell Biol. 108:13-22.

4. Oude Elferink, R.P., J.A. Fransen, J. Klumperman, L.A. Ginsel, and J.M.. Tager. 1989. Secretion of a precursor form of lysosomal alpha-glucosidase from the brush border of human kidney proximal tubule cells. Eur. J. Cell Biol. 50:299-303.

5. Matter, K., B. Stieger, J. Klumperman, L.A. Ginsel, and H.P. Hauri. 1990. Endocytosis, recycling, and lysosomal delivery of brush border hydrolases in cultured human intestinal epithelial cells (Caco-2). J. Biol. Chem. 265:3503-3512.

6. Klumperman, J., J.C. Boekestijn, A.M. Mulder, J.A. Fransen, and L.A. Ginsel. 1991. Intracellular localization and endocytosis of brush border enzymes in the enterocyte-like cell line Caco-2. Eur. J. Cell Biol. 54:76-84.

7. Klumperman, J., J.A. Fransen, J.C. Boekestijn, R.P. Oude Elferink, K. Matter, H.P. Hauri, J.M. Tager, and L.A. Ginsel. 1991. Biosynthesis and transport of lysosomal alpha-glucosidase in the human colon carcinoma cell-line Caco-2: secretion from the apical surface. J. Cell Science 100:339-347.

8. Damke, H., J. Klumperman, K. von Figura, and T. Braulke. 1991. Effects of brefeldin A on the endocytic route. Redistribution of mannose 6-phosphate/insulin-like growth factor II receptors to the cell surface. J. Biol. Chem. 266:24829-24833.

9. Damke, H., J. Klumperman, K. von Figura, and T. Braulke. 1992. Brefeldin A affects the cellular distribution of endocytic receptors differentially. Biochem. Biophys. Res. Comm. 185:719-727.

10. Hille, A., J. Klumperman, H.J. Geuze, C. Peters, F.M. Brodsky, and K. von Figura. 1992. Lysosomal acid phosphatase is internalized via clathrin-coated pits. Eur. J. Cell Biol. 59:106-115.

11. Klumperman, J., J.A. Fransen, J.M. Tager, and L.A. Ginsel. 1992. The cation-independent mannose 6-phosphate receptor is not involved in the polarized secretion of lysosomal alpha-glucosidase from Caco-2 cells. Eur. J. Cell Biol. 57:147-154.

12. Klumperman, J., A. Hille, T. Veenendaal, V. Oorschot, W. Stoorvogel, K. von Figura, and H.J. Geuze. 1993. Differences in the endosomal distributions of the two mannose 6-phosphate receptors. J. Cell Biol. 121:997-1010.

13. Klumperman, J., J. Krijnse-Locker, A. Meijer, M.C. Horzinek H.J. Geuze and P.J. Rottier, 1994. Coronavirus M proteins accumulate in the Golgi complex beyond the site of virion budding. J. Virology 68:6523-6534.

14. Krijnse-Locker, J., J. Klumperman, V. Oorschot, M.C. Horzinek, H.J. Geuze and P.J. Rottier. 1994. The cytoplasmic tail of mouse hepatitis virus M protein is essential but not sufficient for its retention in the Golgi complex. J. Biol. Chem. 269:28263-28269.

15. Itin, C., M. Foguet, F. Kappeler, J. Klumperman, and H.P. Hauri. 1995. Recycling of the endoplasmic reticulum/Golgi intermediate compartment protein ERGIC53 in the secretory pathway. Biochem. Soc. Transactions 23:541-544.

16. Klumperman, J., S. Spijker, J. van Minnen, H. Sharp-Baker, A.B. Smit, and W.P.M. Geraerts. 1996. Cell type specific sorting of neuropeptides: a mechanism to modulate peptide composition of large dense core vesicles. J. of Neuroscience 15:7930-7940.

17. Kuliawat, R., J. Klumperman, L. Ludwig, and P. Arvan. 1997. Differential sorting of lysosomal enzymes out of the regulated secretory pathway in pancreatic beta cells. J. Cell Biol. 137:595-608.

18. Bock, J., J. Klumperman, S. Davanger, and R.H. Scheller. 1997. Syntaxin 6 functions in trans-Golgi network vesicle trafficking. Molc. Biol of the Cell.8:1261-1271.

19. Feng, Z., Klumperman, J., Lukowiak, K. and N.I. Syed. 1997. In vitro synaptogenesis between the somata of identified Lymnaea neurons requires proteins synthesis but not extrinsic growth factors or substrate adhesion molecules. J. Neuroscience 17:7839-7849.

20. Dell’Angelica, E.C., J. Klumperman, W. Stoorvogel, and J.S. Bonaficino. 1998. Association of the AP-3 adaptor complex with clathrin. Science 280:431-434.

21. Klumperman, J., R. Kuliawat, J. Griffith, H.J. Geuze and P. Arvan. 1998. Mannose 6-phosphate receptors are sorted from immature secretory granules via AP-1, clathrin and syntaxin 6-positive vesicles. J. Cell Biol.141:359-371.

22. Hay*, J.C., Klumperman*, J., V. Oorschot, M. Steegmaier, C.S. Kuo and R.H. Scheller. 1998. Localization, dynamics, and protein interactions reveal distinct roles for ER and Golgi SNAREs. J. Cell Biol. 141:1489-1502. *with equal contributions.

23. Klumperman, J., A. Schweizer, H. Clausen, B.L. Tang, W. Hong, V. Oorschot, and H.P. Hauri. 1998. The recycling pathway of protein ERGIC-53 and dynamics of the ER-Golgi intermediate compartment. J Cell Science 111:3411-3425

24. Prekeris, R., J. Klumperman, Y.A. Chen, and R.H. Scheller. 1998. Syntaxin 13 mediates cycling of plasma membrane proteins via tubulovesicular recycling endosomes. J. Cell Biol. 143:957-971.

25. Spencer, GE, J. Klumperman, and NI Syed. 1998. Neurotransmitters and neurodevelopment. Role of dopamine in neurite outgrowth, target selection and specific synapse formation. Perspect Dev Neurobiol. 5:451-467. Review.

26. Chao, D., J.C. Hay, S. Winnick, R. Prekeris, J. Klumperman and R.H. Scheller. 1999. SNARE Membrane trafficking dynamics in vivo. J. Cell Biol. 144:869-881.

27. Steegmaier, M., J. Klumperman, D.L. Foletti, J. Yoo and R.H. Scheller. VAMP4 functions in trans-Golgi network vesicle trafficking. Molc. Biol. of the Cell 10:1957-1972, 1999.

28. Martinez-Menarguez, J., H.J. Geuze, J.W. Slot and J. Klumperman. Vesicular tubular clusters between ER and Golgi mediate concentration of soluble secretory proteins by exclusion from COPI-coated vesicles. Cell 98:81-90, 1999.

29. Advani, R.J., B. Yang, R. Prekeris, K.C. Lee, J. Klumperman, and R.H. Scheller. 1999. VAMP7 mediates vesicular transport from endosomes to lysosomes. J. Cell Biol.146:765-775.

30. de Wit, H., Y. Lichtenstein, H.J. Geuze, R.B. Kelly, P. van der Sluijs and J. Klumperman. 1999. Synaptic vesicles form by budding from tubular extensions of sorting endosomes in PC12 cells. Molc. Biol. of the Cell 10: 4163-4176.

31. Prekeris, R., B. Yang, V. Oorschot, J. Klumperman, and R.H. Scheller. 1999. Differential roles of syntaxin 7 and syntaxin 8 in endosomal trafficking. Molc. Biol. of the Cell 10:3891-3908.

32. Dittie, A., J. Klumperman and S. Tooze. 1999. Mannose-6-phosphate receptors are present on a subset of immature secretory granules in PC12 cells. J. Cell Science 112:3955-3966.

33. Huang, C.S. J. Zhou, A.K. Feng, C.C. Lynch, J. Klumperman, S.J. DeArmond and W.C. Mobley. 1999. NGF signaling in caveolae-like domains at the plasma membrane. J. Biol. Chem. 274: 36707-36714.

34. Krantz, D.E., Y. Liu, R.I. Wilson, P.K. Tan, V. Oorschot, J. Klumperman, and R.H. Edwards. 2000. Phosphorylation regulates sorting of the vesicular acetylcholine transporter to different classes of secretory vesicles. J. Cell Biol. 149:379-395.

35. Steegmaier, M., V. Oorschot, J. Klumperman, and R.H. Scheller. 2000. Syntaxin 17 is abundant in steroidogenic cells and implicated in smooth endoplasmic reticulum membrane dynamics. Molc. Biol. of the Cell. 11:2719-2731.

36. Klumperman, J. 2000. Transport between ER and Golgi. [Review]. Current Opinion in Cell Biology 12:445-449.

37. Prekeris, R., J. Klumperman, and R.H. Scheller. 2000. Syntaxin 11 is an atypical SNARE abundant in the immune system. Eur. J. Cell Biology 79, 771-780.

38. J. Klumperman. The growing Golgi: in search of its independence. 2000. Nature Cell Biol. 2, E217-E218.

39. Prekeris, R., J. Klumperman, and R.H. Scheller. 2000. A Rab11/Rip11 protein complex regulates apical membrane trafficking via recycling endosomes. Molecular Cell 6, 1437-1448.