Saturday, May 15, 2004

Description
HOXB3 TaqI Polymorphism

Procedure
TaqI in HOXB3; Clone pH 2-1-5

Template: Genomic DNA 100-200 ng/reaction volume of 25 祃

Primer 1. H2GCK3 '5-TAT TTT TGT GAT CAA TGG AGA CC 100 ng/reaction
Primer 2. H2GCK3R '5-ACA GAG TCC CTC ACT GCT G 100 ng/reaction

dNTPs: Final Concentration of 200 礛 each

Buffer: Final Concentration 2.25 mM MgCl2, 50 mM KCl, 10 mM Tris HCI pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction volume of 25 祃
(Perkin-Elmer Amplitaq DNA polymerase 5U/祃, Cat No. N808-0145)

PCR Cycling Protocol: 94oC (5')
94oC (30"), 60oC (30"), 72oC (1.0') ?30 cycles

Product Size:
TaqI
316bp ------->200 bp + 116 bp (+/+ individual)

Ogura, T., C.M. Castiglione, A.J. Pakstis, and K.K. Kidd, 1991. Two RFLPs at the HOX2G locus. Nucleic Acids Research 19:1716.


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