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Antigen Retrieval

时间:2006-07-24 14:10来源:Scienceboard.net 作者:admin
Monday, May 03, 2004

Description
Some other useful protocols can be found in the following references:

Caltuvetti et. al., 1993, J. Pathology 171:83 and Shi et. al., 1991 J. Hist. Cytochem. 39:741.

Procedure
Place the slides in a plastic coplin jar filled with denaturing solution and microwave on high for 5 minutes.

?Change the denaturing solution and repeat, cool to room temperature.

?Wash 5 times in PBS and proceed with staining as usual.



Recipes
Solutions

Denaturing Solution

0.8M urea

store at room temperature


Supplies


Tips

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