Monday, May 03, 2004
Description Some other useful protocols can be found in the following references: Caltuvetti et. al., 1993, J. Pathology 171:83 and Shi et. al., 1991 J. Hist. Cytochem. 39:741. Procedure Place the slides in a plastic coplin jar filled with denaturing solution and microwave on high for 5 minutes. Change the denaturing solution and repeat, cool to room temperature. Wash 5 times in PBS and proceed with staining as usual. Recipes Solutions Denaturing Solution 0.8M urea store at room temperature Supplies Tips (责任编辑:泉水) |