Saturday, May 15, 2004

Description
PAH BglII Polymorphism

Procedure
BglII; LOCATED 5' OF EXON 1

Template: Genomic DNA 100-200 ng/reaction volume of 25 l

Primer 1. B2PAH1 5' -GCA GGA AAC TCT CTG ACT TTG G -3' 100 ng/reaction
Primer 2. B2PAH2 5' -TGG CAG TTC TGG AGG CCA GA -3' 100 ng/reaction

dNTPs: Final Concentration 200 M each

Buffer: Final Concentration 1.50 mM MgCl2, 50 mM KCl, 10 mM Tris HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
(Perkin-Elmer Amplitaq DNA polymerase 5 U/l, Cat. No. N808-0145)

PCR Cycling Profile: 95C, 5'
95C (30"), 60C (30"), 72C (30") x 30 cycles

Product Size: BglII
290 bp ------->209 bp and 81 bp (+/+ individual)


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