5% Uranyl Acetate Solution:
To prepare 50 ml,
Uranyl acetate --------------------- 2.5 g
Distilled water --------------------- 50 ml
Cover with foil and stir overnight.
Add 10 drops of glacial acetic acid and Store solution in 4 C for 3 month.
Reynold’s Lead Citrate Solution:
To prepare 50 ml, add chemicals in distilled water in following order
Lead nitrate ------------------------------- 1.33 g
Sodium citrate, dihydrate -------------- 1.76 g (solution becomes cloudy when sodium citrate is added)
1N NaOH ----------------------------------- 5 ml (solution becomes clear when NaOH is added)
Distilled water ----------------------------- 30 ml
Stir for 10 minutes to dissolve and add additional 15 ml of distilled water. Store solution for 3-6 months at 4 C. Note: the mount of NaOH is very important. Too much will make solution cloudy.
Epon 812:
|
Final Volume |
EM bed-812 |
DDSA |
NMA |
DMP-30 (2%) |
|
20.91 ml |
10 ml |
4.5 ml |
6 ml |