- Fertilization of frog eggs
- Primers for RT-PCR
- Frog whole mount in situ hybridization
- Mouse whole mount in situ hybridization
- Paraplast in situ on sections
- Cryostat in situ on sections
- Skeletal staining
- Alcian blue cartilage staining
- RT-PCR
- Animal Cap Assay
- Dissociation and reaggregation of animal cap cells
- Keller sandwich explant
- Spemann's organizer transplantation
- BDA lineage tracing on sections
- Whole-mount lacZ lineage tracing
- Hematoxilin and eosin staining
- Whole-mount staining with Alkaline phosphotase antibodies
- Natural and artificial fertilization
- Western blot for phosphoprotein samples
- Northern blot - Hiroki Kuroda
- In situ hybridizations on sections
De Robertis' Laboratory protocals
核心摘要：
本实验室方法涵盖多种胚胎发育研究技术，包括青蛙及小鼠全胚体和切片原位杂交、RT-PCR扩增、动物帽细胞的解离重聚及动物帽测定，结合Spemann组织移植和BDA及lacZ谱系追踪，系统解析胚胎细胞命运及信号传导机制。此外，采用自然及人工受精技术，辅以蛋白质磷酸化的Western blot和核酸Northern blot分析，构建多层次分子与细胞发育调控体系。