-
[DNA Library] APOB EcoRI Polymorphism 日期：2005-07-18 00:00:00 点击：269 好评：0
Saturday, May 15, 2004 Description APOB EcoRI Polymorphism Procedure EcoR I Template: Genomic DNA 100-200 ng/reaction volume of 25 l. Primer 1. APOBEC1 5'-CTG GCT TGC TAA CCT CTC TG-3' 100 ng/Reaction Primer 2. APOBEC2 5'-GAG AAG CTT CCT G...
-
[DNA Library] Construction of a genomic DNA library 日期：2005-07-18 00:00:00 点击：254 好评：0
Thursday, December 04, 2003 Description Construction of a genomic DNA library Procedure Library construction Two micrograms of genetically modified genomic tobacco plant DNA was digested with 60 units of the restriction enzyme, BamHI and l...
-
[DNA Library] Agrobacterium Transformation 日期：2005-07-18 00:00:00 点击：271 好评：0
Description Agrobacterium Transformation Procedure 1. Seed culture in YEP/Streptomycin+ media, 28, shaking for overnight 2. Add 3-4 mL of overnight cultured cell to 50 mL of fresh YEP/Streptomycin+ media, 4 hr shaking (OD6000.5) 3. 4000 rp...
-
[DNA Library] AGROBACTERIUM VIR GENE INDUCTION PROTOCOL 日期：2005-07-18 00:00:00 点击：200 好评：0
Thursday, December 04, 2003 Description AGROBACTERIUM VIR GENE INDUCTION PROTOCOL Procedure 1. Grow Agrobacterium at 30oC overnight in 5 ml rich medium (such as LB or YEP) containing the appropriate antibiotics. 2. Dilute approximately 0.5...
-
[DNA Library] TOMATO TRANSFORMATION 日期：2005-07-18 00:00:00 点击：286 好评：0
Thursday, December 04, 2003 Description TOMATO TRANSFORMATION Procedure Sterilize dry seeds in 70% ethanol - 2 min. followed by 3.3% NaOCl (1:3 dilution of 10%) with 0.1% Tween 20 for 20 min., in an 250 ml Erlenmeyer. Stir fast with magnet...
-
[DNA Library] BIBAC LIBRARY Agrobacterium plasmid prep 日期：2005-07-18 00:00:00 点击：288 好评：0
Thursday, December 04, 2003 Description Following this protocol, large single copy plasmids (including Ti plasmids) will be isolated as well as any higher copy and/or smaller plasmids. Since in most cases the binary plasmid is higher copy...
-
[DNA Library] [BIBAC] Electroporation protocols 日期：2005-07-18 00:00:00 点击：241 好评：0
Thursday, December 04, 2003 Description A binary-BAC (BIBAC) vector suitable for Agrobacterium-mediated plant transformation with high-molecular-weight DNA. A BIBAC vector is based on the bacterial artificial chromosome (BAC) library vecto...
-
[DNA Library] Subcloning inserts from traditional BAC or PAC vectors into 日期：2005-07-18 00:00:00 点击：215 好评：0
Thursday, December 04, 2003 Description A binary-BAC (BIBAC) vector suitable for Agrobacterium-mediated plant transformation with high-molecular-weight DNA. A BIBAC vector is based on the bacterial artificial chromosome (BAC) library vecto...
-
[DNA Library] BIBAC library ISOLATION OF PLASMID DNA 日期：2005-07-18 00:00:00 点击：268 好评：0
Thursday, December 04, 2003 Description A binary-BAC (BIBAC) vector suitable for Agrobacterium-mediated plant transformation with high-molecular-weight DNA. A BIBAC vector is based on the bacterial artificial chromosome (BAC) library vecto...
-
[DNA Library] Cosmid library-Large -scale prep of cosmids 日期：2005-07-18 00:00:00 点击：209 好评：0
Thursday, December 04, 2003 Description The library is a L. pennellii library . The cosmid library was made from MboI partially digested total genomic DNA which was size fractionated on a sucrose gradient and selected for a range of approx...