|
Saturday, May 15, 2004
Description DRD2 HincII Polymorphism Procedure Hinc II polymorphism in DRD2 located 1.9 kb downstream from exon 8 Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. D2EX8F9: 5' -GAG CAA TCT TGT GCT CCA G-3' 100 ng/reaction Primer 2. D2Hinc2R: 5' -GGA GAA AGG TGC CTT GGA AG-3' 100 ng/reaction dNTPs: Final Concentration 200 M each Buffer: Final Concentration 1.5 mM MgCl2, 50 mM KCl, 10 mM Tris HCl pH 8.4 Taq DNA Polymerase: 0.5 Units/reaction (Perkin-Elmer Amplitaq DNA polymerase 5 U/ l, Cat. No. N808-0145) PCR Cycling Profile: 94C (5') 94C (30"), 60C (30"), 72C (1.0') x 30 cycles Product size: Hinc II 190 bp > 140 bp + 50 bp (in homozygote, site present) Recipes Supplies Tips (责任编辑：泉水) |
DRD2 HincII Polymorphism
时间:2005-07-18 00:00来源:Scienceboard.net 作者:admin 点击:
274次
顶一下
(5)
100%
踩一下
(0)
0%
------分隔线----------------------------
- 发表评论
-
- 最新评论 进入详细评论页>>
