Saturday, May 15, 2004
Description NGFR HindIII Polymorphism Procedure HindIII (PCR location in AC006487: 804-1295 bp; HindIII location in AC006487: 969-974 bp) Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. NGFRHND3F 5' CCC CTT GCGG ATG TTC TC 3' 90ng/reaction Primer 2. NGFRHND3R 5' GTG GGA TGG CTG GAC CTG 3' 90 ng/reaction dNTPs: Final Concentration 200 M each Buffer: Final Concentration 1.5 mM MgCl2, 50 mM KCl, 10 mM Tris HCl pH 8.4 Taq DNA Polymerase: 0.5 Units/reaction (Perkin-Elmer Amplitaq DNA polymerase 5 U/l, Cat. No. N808-0145) PCR Cycling Profile: 94C, 5 min 94C (30"), 58C (30"), 72C (30") x 30 cycles 72C (7') Product size: HindIII 492------------------> 324 and 168 bp in heterozygote Recipes Supplies Tips (责任编辑:泉水) |