|
Saturday, May 15, 2004
Description SLC6A3 3' VNTR Polymorphism Procedure 40-bp VNTR located in 3'-UTR (422 bp downstream from G2319A MspI polymorphism) Template: Genomic DNA 100-200 ng/reaction volume of 20 L Primer 1. DATVNTRF 5'-TGTGGTGTAGGGAACGGCCTGAG-3' (.2 M) Primer 2. DATVNTRR 5'-CTTCCTGGAGGTCACGGCTCAAGG-3' (.2 M) dNTPs: Final concentration 200 M Buffer: Final Concentration 1.5 mM MgCl2, 50 mM KCl, 10 mM Tris-HCl pH 8.3 DMSO: Final Concentration 5% Taq DNA Polymerase: 1.0 U/reaction PCR Cycling Profile: 95C, 5' 94C (30"), 62C (30"), 72C (30") x 30 Product Size: 564 bp 12 repeats 524 bp 11 repeats 2% agarase gel, EtBr stain 484 bp 10 repeats 444 bp 9 repeats 364 bp 7 repeats 199 bp 3 repeats Recipes Supplies Tips (责任编辑：泉水) |
SLC6A3 3 VNTR Polymorphism
时间:2005-07-18 00:00来源:Scienceboard.net 作者:admin 点击:
300次
顶一下
(0)
0%
踩一下
(0)
0%
------分隔线----------------------------
- 发表评论
-
- 最新评论 进入详细评论页>>
