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Saturday, May 15, 2004
Description PAH PvuII A Polymorphism Procedure PvuII A; LOCATED NEAR THE 5' END OF INTRON 2 Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. PAHPVU2-1 5 -GGC ATG ACT GGA TAC GAT TAG-3' 100 ng/reaction Primer 2. PAHPVU2-2 5 -CTA GAC TCA GAA TGC CTG GG -3' 100 ng/reaction dNTPs: Final Concentration 200 M each Buffer: Final Concentration 1.50 mM MgC12, 50 mM KCl, 10 mM Tris HCl pH 8.4 Taq DNA Polymerase: 0.5 Units/reaction (Perkin-Elmer Amplitaq DNA polymerase 5 U/l, Cat. No. N808-0145) PCR Cycling Profile: 95C, 5' 95C (30"), 58C (30"), 72C (30") x 30 cycles Product size: PvuII 374 bp ----------->225 bp and 149 bp (+/+ individual) Recipes Supplies Tips (责任编辑：泉水) |
PAH PvuII A Polymorphism
时间:2005-07-18 00:00来源:Scienceboard.net 作者:admin 点击:
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