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[PCR] Leaf GUS Assay 日期：2005-07-18 00:00:00 点击：229 好评：0
Monday, November 10, 2003 Description Leaf GUS Assay Procedure This protocol is for small samples (usually single leaf from 21DAI plants), scale up for larger samples As there are usually many samples, we use a microtitre plate and plate r...
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[PCR] Inverse PCR Protocol 日期：2005-07-18 00:00:00 点击：421 好评：0
Monday, November 10, 2003 Description Inverse PCR Protocol This method can be used directly for pBIN19 or pBI101 based T-DNA inserts Procedure Digestion reaction: 2.5g genomic DNA (CTAB) 1x 10x enzyme buffer 20-40U restriction enzyme xl dd...
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[PCR] Competitive Ligand Binding to Cortical Receptor using Crossl 日期：2005-07-18 00:00:00 点击：297 好评：0
Sunday, October 19, 2003 Description This protocol describes a competitive ligand binding assay for cortical neurotrophin receptors. Following binding in the presence of competitor, the bound radiolabeled ligand is cross-linked to the rece...
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[PCR] Gel Mobility Shift Assay Using Magnesium Chloride and EDTA 日期：2005-07-18 00:00:00 点击：324 好评：0
Sunday, October 19, 2003 Description Gel Mobility Shift Assay Using Magnesium Chloride and EDTA Procedure A. Gel Shift Reaction 1. Prepare a 20 l binding reaction by combining the following ingredients: 4 l of 5X Binding Buffer 0.2 l of 0....
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[PCR] Beta-Lactamase Induction (Crude Preparation) 日期：2005-07-18 00:00:00 点击：269 好评：0
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