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[PCR] PCR Transformation 日期：2006-08-17 06:28:24 点击：463 好评：0
PCR Transformation Grow cells to an OD600 0.7-2.5 (25ml culture) Spin cells 4500 rpm for 2 min Resuspend in 50 ml 0.1M LiOAc Repeat step 2 and 3 Resuspend in 0.1M LiOAc (culture volume x OD/100) Add 1/9 volume of 10 mg/ml ssDNA that has be...
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[PCR] DNA precipitation 日期：2005-07-18 00:00:00 点击：375 好评：0
Monday, June 07, 2004 Description This protocol describes a method to precipitate DNA in order to concentrate the DNA fragment or plasmid fo further downstream processes. Procedure 1 Extract DNA fragment following PCR reaction using a comm...
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[PCR] Mini prep and restriction digest conformation 日期：2005-07-18 00:00:00 点击：361 好评：0
Monday, June 07, 2004 Description This precedure describes a method for preparation of plasmid DNA and confirmation by restriction endonuclease digestion. See also protocol for cloning of DNA fragments. Procedure 1 Grow bacterial colonies...
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[PCR] Cloning of DNA fragments 日期：2005-07-18 00:00:00 点击：334 好评：0
Monday, June 07, 2004 Description This protocol describes the various steps involved in clonig a DNA fragment into a vector fro production of a plasmid via bacterial cells. This cincudes teh cDNA synthesis, PCR reaction, ligation reaction...
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[PCR] Differential Display PCR 日期：2005-07-18 00:00:00 点击：370 好评：0
Friday, June 04, 2004 Description To identify and characterize those genes that are differentially expressed in order to understand the molecular nature of disease state and subsequently, to devise rational therapies. Differential display...
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[PCR] RAPD ( Randomly Amplified Polymorphic DNA) 日期：2005-07-18 00:00:00 点击：198 好评：0
Friday, June 04, 2004 Description RAPD ( Randomly Amplified Polymorphic DNA) analysis with 'P. infestans' Procedure Reaction conditions: 25 ul per reaction sterile di water 16 ul 1O X buffer 2.5 ul 100 mM KCI 200 mM Tris-HCI (pH 8.8) 100 m...
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[PCR] PCR of GC rich templates 日期：2005-07-18 00:00:00 点击：402 好评：0
Sunday, February 01, 2004 Description High GC content can prohibit the PCR reaction due to improper denaturing of the template or annealing of the primers. This minor spin on a common protocol is simple but works incredibly well. Procedure...
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[PCR] Tail PCR 日期：2005-07-18 00:00:00 点击：600 好评：0
Thursday, December 04, 2003 Description Tail PCR Procedure Amplification of flanking sequences To determine flanking sequences of RDA subtraction products in the genome, a two-step PCR reaction technique can be applied using a biotinylated...
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[PCR] OLIGO PURIFICATION on acrylamide gels 日期：2005-07-18 00:00:00 点击：314 好评：0
Friday, November 21, 2003 Description OLIGO PURIFICATION on acrylamide gels Procedure GEL PURIFICATION OF OLIGONUCLEOTIDES 1. Quantitate crude oligo solution via UV spectrophotometry. Assume 1.0 A260 unit is equal to 33 ug/ml. Dry down 100...
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[PCR] PRIMER LABELLING FOR PRIMER EXTENSION ASSAY and PRIMER EXTEN 日期：2005-07-18 00:00:00 点击：368 好评：0
Friday, November 21, 2003 Description PRIMER LABELLING FOR PRIMER EXTENSION ASSAY and PRIMER EXTENSION Procedure Mix in a 20ul final volume: 2ul 10x kinase buffer 1ul oligonucleotide primer (5pmoles/ul) 200uCi 32P-gATP (crude, ICN, 7000Ci/...