This protocol has been optimized for 6 – 10 dpc embryos. Important points: Embryos should be dissected and quickly transferred to fixative as phosphorylation of MAPK is very labile. Fixation must be enough to preserve the diphosphorylatio...
Dissect embryos in cold PBS containing 2 mM EGTA. Day 9 embryos and older should have the brain punctured to allow solutions in and out. Fix embryos in 10-15 ml of fresh, cold 4% paraformaldehyde/2 mM EGTA in PBS for two hours at 4°C with...
Mouse 15K microarray hybridization protocol protocol by Dennis Wigle last updated May 2002 (modified from Niki Dobric the OCI array facility) NB: It is very important to order and prepare all reagents, and review recipes before beginning p...
Fertilization of frog eggs Primers for RT-PCR Frog whole mount in situ hybridization Mouse whole mount in situ hybridization Paraplast in situ on sections Cryostat in situ on sections Skeletal staining Alcian blue cartilage staining RT-PCR...
来自纽约2月13日的消息，美国能源部联合基因组研究所（DepartmentofEnergy'sJointGenomeInstitute，DOEJGI）的研究人员表示认为在微生物基因组测序方面表现出巨大的潜力的454LifeSciences公司的测序仪（见快速基因组测序时代到来）虽然并不能完全取代传统的Sa...
自20世纪70年代中期首例cDNA克隆问世以来，构建cDNA文库已成为研究功能基因组学的基本手段之一。cDNA便于克隆和大量表达，它不像基因组含有内含子而难于表达，因此可以从cDNA文库中筛选到所需的目的基因，并直接用于该目的基因的表达。通过构建cDNA表达文库...
这是最早用于性病诊断的重组DNA技术。基本原理...
一、概述 近年来核酸杂交技术在分子生物学、临...
巢式PCR的定义： 巢式PCR是一种变异的聚合酶链反...
很好的一篇关于RNA抽提的综述文章。 对大部分实...
