PAH MspI Polymorphism

Saturday, May 15, 2004

Description
PAH MspI Polymorphism

Procedure
MspI; LOCATED NEAR THE 5' END OF INTRON 8

Template: Genomic DNA 100-200 ng/reaction volume of 25 l

Primer 1. PAHM 5 -GAA GCA TAT TGT ATC TGC CC -3' 100 ng/reaction
Primer 2. PAHM2 5' -CAC ATG TCC CAA CAG CTC AT -3' 100 ng/reaction

dNTPs: Final Concentration 200 M each

Buffer: Final Concentration 2.25 mM MgC12, 50 mM KCl, 10 mM Tris HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
(Perkin-Elmer Amplitaq DNA polymerase 5 U/ l, Cat. No. N808-0145)

PCR Cycling Profile: 95C, 5'
95C (30"), 58C (30"), 72C (30") x 30 cycles

Product size: MspI
425 bp ----------->300 bp and 125 bp (+/+ individual)

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PAH MspI Polymorphism