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[General Lab] Fluorescent - Auramine Rhodamine Staining For Mycobacterium 日期：2005-07-18 00:00:00 点击：192 好评：0
Friday, November 21, 2003 Description The best known and distinctive property of the genus, Mycobacteria, depends upon their lipid-rich cell walls which are relatively impermeable to various basic dyes unless the dyes are combined with phe...
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[General Lab] SMEAR PREPARATION 日期：2005-07-18 00:00:00 点击：181 好评：0
Friday, November 21, 2003 Description The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being...
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[General Lab] Gram-staining Procedure 日期：2005-07-18 00:00:00 点击：229 好评：0
Friday, November 21, 2003 Description Gram-staining is a four part procedure which uses certain dyes to make a bacterial cell stand out against against its background. The specimen should be mounted and fixed on a slide before you procede...
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[General Lab] PASSIVE AGGLUTINATION 日期：2005-07-18 00:00:00 点击：284 好评：0
Friday, November 21, 2003 Description Agglutination Assay Procedure Passive agglutination is performed using tanned sheep red blood cells and P. aeruginosa LPS (heated for 1 hour at 100oC prior to use). Sheep erythrocytes are washed three...
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[General Lab] BACTERIAL AGGLUTINATION: 日期：2005-07-18 00:00:00 点击：199 好评：0
Friday, November 21, 2003 Description Agglutination Assay Procedure Bacterial agglutination is performed using either P. aeruginosa cells autoclaved at 120oC for 1 hour, then centrifuged and resuspended in saline or live P. aeruginosa cell...
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[General Lab] Terminal dUTP Nick End-Labeling (TUNEL) of Barley Aleurone P 日期：2005-07-18 00:00:00 点击：299 好评：0
Friday, November 21, 2003 Description Degradation of nuclear DNA can be examined by the TUNEL assay, which allows for in situ detection of nicked DNA and DNA breaks at the single cell level. This method is based on the enzymatic labeling w...
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[General Lab] Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction 日期：2005-07-18 00:00:00 点击：239 好评：0
Friday, November 21, 2003 Description Protocol for Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction Procedure 1. Grind 2 to 5 g of frozen leaves to a very fine powder with a liquid nitrogen-cooled mortar and pestle. 2. Add 25 ml...
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[General Lab] Isolation of RNA from Tobacco Mosaic Virus (TMV) 日期：2005-07-18 00:00:00 点击：173 好评：0
Friday, November 21, 2003 Description TMV is a helical rod with two components, an RNA of 6.1 kb and a protein of 17.5 kDa. Ninety-five percent of the mass is protein and 5% of the mass is RNA. To isolate the RNA you dissociate the two com...
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[General Lab] Determination of Chlorophyll Content in Chloroplasts 日期：2005-07-18 00:00:00 点击：227 好评：0
Friday, November 21, 2003 Description This protocol describes how to determine the chlorophyll content of chloroplasts using a spectrophotometric assay. Procedure 1. Isolate Chloroplasts from spinach leaves (See protocol for preparation of...
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[General Lab] Demonstration of endospores by means of the Shaeffer-Fulton 日期：2005-07-18 00:00:00 点击：164 好评：0
Thursday, November 20, 2003 Description Please see the procedure Procedure 1. Flame one side of the slide in the flame of your Bunsen burner to remove any oil or grease. Allow the slide to cool. 2. Place a drop of water in the center of th...