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[Immunology] ELISA for IFN-gamma and IL-10 日期：2005-07-18 00:00:00 点击：210 好评：0
Wednesday, October 29, 2003 Description A simple ELISA assay to measure cytokines in culture supernatants Procedure -Coat plates with primary Antibody 2 g/ml, 50 l/well -Incubate overnight 4C -Wash 3 times -Block with 10% FCS, 200 ml/well...
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[Immunology] Antigen-specific intracellular Calcium flux measurement 日期：2005-07-18 00:00:00 点击：198 好评：0
Wednesday, October 29, 2003 Description A method to measure Ag-specific Calicium flux in mice cells. After immunization of mice lymphocytes are collected and restimulated in vitro. In parallel, antigen-presenting cells are loaded with the...
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[Immunology] JNK/SAP Kinase Assay 日期：2005-07-18 00:00:00 点击：271 好评：0
Monday, October 27, 2003 Description JNK/SAP Kinase Assay Procedure A. Stimulation and Harvesting of Cells 1. Grow cells to confluence in 100 mm culture dishes. 2. After cells reach confluence, grow in serum-free media for up to 48 hours....
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[Immunology] Raf-1 Kinase Assay 日期：2005-07-18 00:00:00 点击：220 好评：0
Monday, October 27, 2003 Description This protocol uses mitogen-activated protein/ERK kinase (MEK) to activate the extracellular-signal-regulated (Erk) mitogen-activated protein (MAP) kinases upon agonist binding to receptors. Procedure A....
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[Immunology] Telomeric Repeat Amplification Protocol 日期：2005-07-18 00:00:00 点击：336 好评：0
Description This protocol describes a semi-quantitative in vitro assay to detect telomerase activity. Any active telomerase present in a cell extract from detergent-lysed cells will add a variable number of telomeric repeats (TTAGGG) to th...
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[Immunology] Quantitative Fluorescent Assay for the Simultaneous Identifi 日期：2005-07-18 00:00:00 点击：221 好评：0
Monday, October 27, 2003 Description The purpose of this fluorescent method is to simultaneously measure apoptotic cells, necrotic cells, normal (non-apoptotic/non-necrotic) cells, and apoptotic cells that have undergone secondary necrosis...
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[Immunology] CPP32 Activity Assay Using CPP32 Fluorogenic Substrate 日期：2005-07-18 00:00:00 点击：194 好评：0
Monday, October 27, 2003 Description CPP32 Activity Assay Using CPP32 Fluorogenic Substrate Procedure . Determine the protein concentration of the sample to be analyzed (see protocol on Quantification of Protein Samples). For each reaction...
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[Immunology] The Under-Agarose Migration Assay 日期：2005-07-18 00:00:00 点击：164 好评：0
Description The Under-Agarose assay is a useful method for observing the response of a cell population to one or more chemoattractant sources. The behavior of individual migrating cells can be studied by modifying the assay for video micro...
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[Immunology] Bioassay IFN-gamma 日期：2005-07-18 00:00:00 点击：212 好评：0
Saturday, October 18, 2003 Description Bioassay for IFN-gamma Procedure 1) Prepare L929 or A549 cell suspension at a density of 3.5 x 105/ml in assay medium. Add 100 l/well to the 96-well plate and incubate overnight at 37 degrees C, 5% CO...
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[Immunology] Immunodetection of cyclin D1 and D2/D3 using flow cytometry 日期：2005-07-18 00:00:00 点击：202 好评：0
Saturday, October 18, 2003 Description This protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specifi...