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Tuesday, March 16, 2004
Description This procedure describes the freezing of the rodent cells with serum. Procedure Filter sterilize 700ml of RPMI-1640 (2mM L-Glutamine) and 200ml fetal bovine serum (FBS) through a 0.22m filter. Add 100ml sterile dimethyl sulfoxide (DMSO). Aliquot into tubes as appropriatePrepare freezing medium as indicated . Harvest cells in log phase growth. Count cells be accepted method and centrifuge.Suspend the cells in the freezing medium at 5x106 cells/mL. Aliquot the cells as appropriate. Freeze the cells in a rate controlled freezer or by placing them at -20oC for approximately 3 hours and at -70oC overnight. Store cells in liquid nitrogen. Recipes Supplies Tips (责任编辑：泉水) |
Freezing of Cells with Serum
时间:2006-07-24 14:26来源:Scienceboard.net 作者:admin
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