Tuesday, June 29, 2004 Description This protocol describes how to freeze tissue culture cells (mamalian) in a way that they will be viable when thawed. Procedure 1.) Aspirate media 2.) Wash cells with PBS 3.) Apply trypsin/EDTA (1ml for 15...
Saturday, April 30, 2005 Description A method to prepare large ammounts or synthetic RNA using T7, T3 or SP6 RNA polymerases. Procedure Template Preparation and RNA Transcription PCR generated templates or 2 x Cesium Cl2 banded plasmid DNA...
Wednesday, May 04, 2005 Description Allows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation. Procedure 1. Grow the cells in regular media to 70% confluence and treat the cel...
Wednesday, May 18, 2005 Description Protocol for rapid extraction of undegraded proteins from cultured human monocytes for the purpose of immunoblotting Procedure Isolate PBMCs from Buffy Coats as usual, and select CD14+ monocytes using an...
Wednesday, June 01, 2005 Description Fluorescence based asssay for establishing neuronal viability in cell culture monolayers. Procedure Monolayer cultures of rat embryonic cortical neurons were prepared and plated at a density of 2-5 x 10...
Sunday, June 12, 2005 Description Procedure for culturing and maintaining Dorsal Root Ganglion (DRG) neurons from adult rats. Procedure Preparing Culture Surface -Collagen coat cover slips (if using) or cell culture dishes: evenly spread 2...
Tuesday, June 14, 2005 Description We maintain the stock of 3T3-L1 cells in 150 mm tissue culture dishes in DMEM (Gibco catalog no. 11995-065) supplemented with 10% Fetal Bovine Serum, 100 units/ml of Penicillin and 100 礸/mL of Streptomyc...
A Simple Method for Collecting Embryos from Xenopus tropicalis By Vladimir S. Apekin /Sive Lab Introduction The small size of the X. tropicalis makes the use of the traditional X. laevis methods of embryo collection very difficult. Therefo...
Anatomy of the 24, 48, 72 and 120 hours Zebrafish ( Danio rerio ) Embryo This collection of sections through zebrafish embryos at four different stages of development is thought to provide some help to understand how the zebrafish embryo l...
A guide for the laboratory use of zebrafish Danio...