-
[Cell Culture] Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF 日期：2006-07-24 14:10:46 点击：30 好评：0
Monday, May 03, 2004 Description Weskamp, G., and Otten, U. (1987). An enzyme-linked immunoassay for nerve growth factor (NGF): A tool for studying regulatory mechanisms involved in ngf production in brain and in peripheral tissues. Journa...
-
[Cell Culture] Dissociated Retina Immunohistochemistry 日期：2006-07-24 14:10:45 点击：43 好评：0
Monday, May 03, 2004 Description Dissociated Retina Immunohistochemistry Procedure before starting see criteria/guidelines for dissociated cell scoring ?Wash the tissue (freshly dissected or explant) in prewarmed PBS (6 ml in a 6 cm dish)....
-
[Cell Culture] Antigen Retrieval 日期：2006-07-24 14:10:45 点击：90 好评：0
Monday, May 03, 2004 Description Some other useful protocols can be found in the following references: Caltuvetti et. al., 1993, J. Pathology 171:83 and Shi et. al., 1991 J. Hist. Cytochem. 39:741. Procedure Place the slides in a plastic c...
-
[Cell Culture] PEROXIDASE-ANTI-PEROXIDASE TECHNIQUE FOR THE IDENTIFICATION 日期：2006-07-24 14:10:44 点击：29 好评：0
Monday, May 03, 2004 Description PEROXIDASE-ANTI-PEROXIDASE TECHNIQUE FOR THE IDENTIFICATION OF ANTIGENS IN PARAFFIN WAX SECTIONS Procedure Dewax sections and transfer to 100% alcohol. Block endogenous peroxidase with 0.5% hydrogen peroxid...
-
[Cell Culture] Cytokine-Induced Proliferation of Indicator Cell Lines 日期：2006-07-24 14:10:44 点击：30 好评：0
Monday, May 03, 2004 Description Cytokine-Induced Proliferation of Indicator Cell Lines Procedure Materials Indicator cell line (see Quick Guide Chart for a given cytokine) Culture Medium (RPMI-1640 supplemented with 10% FBS) 96-well flat-...
-
[Cell Culture] TNF-alpha-Induced Killing of L929 Cell Line 日期：2006-07-24 14:10:43 点击：77 好评：0
Monday, May 03, 2004 Description TNF-alpha-Induced Killing of L929 Cell Line Procedure Materials L929 mouse fibroblast line (ATCC Cat. No. CCL-1) Culture Medium (RPMI supplemented with 10% FBS) Assay Medium (RPMI supplemented with 2% FBS)...
-
[Cell Culture] IFN-gamma Protection from Viral Infection of Cell Lines 日期：2006-07-24 14:10:43 点击：27 好评：0
Monday, May 03, 2004 Description IFN-gamma Protection from Viral Infection of Cell Lines Procedure Materials L929 mouse fibroblast line (ATCC Cat. No. CCL-1) or A549 human lung carcinoma (ATCC Cat. No. CCL-185) Culture Medium (RPMI supplem...
-
[Cell Culture] Cytokine ELISPOT Protocol 日期：2006-07-24 14:10:42 点击：50 好评：0
Monday, May 03, 2004 Description ELISPOT (Enzyme-linked ImmunoSPOT) technique was originally used to enumerate antibody secreting B cells. In the current technique, cells are deposited onto a membrane coated with one antibody specific for...
-
[Cell Culture] Antibody Neutralization of Cytokine-induced Proliferation of 日期：2006-07-24 14:10:42 点击：22 好评：0
Monday, May 03, 2004 Description Antibody Neutralization of Cytokine-induced Proliferation of Indicator Cell Lines Procedure Materials Indicator cell line (see Quick Guide Chart for a given cytokine) Culture Medium (RPMI supplemented with...
-
[Cell Culture] Antibody Neutralization of TNFa-Induced Killing of L929 Cell 日期：2006-07-24 14:10:41 点击：34 好评：0
Monday, May 03, 2004 Description Antibody Neutralization of TNFa-Induced Killing of L929 Cell Line Procedure Materials L929 mouse fibroblast line (ATCC Cat. No. CCL-1) Culture Medium (RPMI supplemented with 10% FBS) Assay Medium (RPMI supp...