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[Cell Culture] G1/S cell synchnronization using double thymidine block 日期：2006-07-24 14:10:51 点击：132 好评：0
Thursday, January 29, 2004 Description This protocol is designed to synchronize HeLa cells at the G1/S border (actually all G1). It is highly effect and gives almost complete synchronization in the culture. Note that this protocol will not...
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[Cell Culture] Wrights staining 日期：2006-07-24 14:10:50 点击：51 好评：0
Tuesday, March 16, 2004 Description Wright's stain is a Romanowsky type metachromatic stain made by mixing old or specially treated methylene blue dye with eosin in a methanol diluent. Basic components of the cell, such as hemoglobin or ce...
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[Cell Culture] Cell counts 日期：2006-07-24 14:10:50 点击：71 好评：0
Tuesday, March 16, 2004 Description When one refers to particle counting in the clinical laboratory, he or she is usually referring to the counting of blood cells. Cells are, in fact, particles, and these particles are suspended in a liqui...
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[Cell Culture] S閦ary Cell Preparation 日期：2006-07-24 14:10:49 点击：42 好评：0
Tuesday, March 16, 2004 Description S閦ary cells are peripheralized malignant T cells seen in some patients with mycosis fungoides. Peripheral blood smears are stained with Wright-Giemsa stain and evaluated by the director of the laborator...
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[Cell Culture] Freezing of Cells with Serum 日期：2006-07-24 14:10:49 点击：30 好评：0
Tuesday, March 16, 2004 Description This procedure describes the freezing of the rodent cells with serum. Procedure Filter sterilize 700ml of RPMI-1640 (2mM L-Glutamine) and 200ml fetal bovine serum (FBS) through a 0.22祄 filter. Add 100ml...
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[Cell Culture] Culture of Dissociated Sympathetic Neurons 日期：2006-07-24 14:10:48 点击：16 好评：0
Monday, May 03, 2004 Description Culture of Dissociated Sympathetic Neurons Procedure 1. Use day 9 chicken embryos. 2. Dissect sympathetic chains in Ham's F12 media. 3. Dissociate ganglia through incubation with 0.25% trypsin for 20 min at...
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[Cell Culture] Culture of Dissociated Cortical Neurons 日期：2006-07-24 14:10:48 点击：19 好评：0
Monday, May 03, 2004 Description Culture of Dissociated Cortical Neurons Procedure 1. Use 7 to 8 day chicken embryos 2. Dissect forebrain in Ham's F12 media, peel off all meninges and then separate cortex from basal ganglia 3. Dissociate n...
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[Cell Culture] Conditioned Medium for Retinal Explant Culture 日期：2006-07-24 14:10:47 点击：55 好评：0
Monday, May 03, 2004 Description Conditioned Medium for Retinal Explant Culture Procedure Remove E14.5 rat retinae with lens intact in prewarmed explant culture medium. Immediately place 4 retinae on a polycarbonate filter (1 micron pore s...
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[Cell Culture] Organotypic Culture 日期：2006-07-24 14:10:47 点击：72 好评：0
Monday, May 03, 2004 Description Organotypic Culture Procedure Organotypic Hippocampal Slice Cultures 1. MED probe coating: Polyethylenimine (PEI) method a. Sterilize the MED probe with 70% ethanol (DO NOT autoclave). b. Dry up the MED pro...
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[Cell Culture] Preparation of a dispersed neuron culture of rat suprachiasm 日期：2006-07-24 14:10:46 点击：27 好评：0
Monday, May 03, 2004 Description Preparation of a dispersed neuron culture of rat suprachiasmatic nucleus (SCN) Procedure Rat pups were anesthetized by hypothermia and decapitated. Frontal sections of the hypothalamus (700 祄 thick) were o...