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[Cell Culture] Rice Bombardment 日期：2006-07-24 14:10:56 点击：28 好评：0
Thursday, December 04, 2003 Description Rice Bombardment Procedure DAY 1 1. Filtering Rice cells with 640 兪m mesh to get small size cells. 2. Discarding the sup 3. Mountaining #4 whatman paper on the N6D 0.5 media 4. Spread 250mg cell on...
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[Cell Culture] PP2C assay 日期：2006-07-24 14:10:55 点击：67 好评：0
Thursday, December 04, 2003 Description PP2C assay Procedure 1. Phosphorylation of casein Casein kinaseI (NEB), 1000U/ul Sigma casein C-4765, 5% solution (56mg/ml), dilute to 10mg/ml 500ul reaction, 30oC, 4h 50 mM Tris-HCl, pH 7.5 10 mM Mg...
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[Cell Culture] Transient Expression Assay Using Arabidopsis Mesophyll Proto 日期：2006-07-24 14:10:55 点击：73 好评：0
Thursday, December 04, 2003 Description Transient Expression Assay Using Arabidopsis Mesophyll Protoplasts : Protoplast Isolation Procedure Plant Materials: BE plants grown on the B5 medium Greenhouse-grown BE, Col, Ler and C24 plants are...
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[Cell Culture] Transient Expression Assay Using Arabidopsis Mesophyll Proto 日期：2006-07-24 14:10:54 点击：70 好评：0
Thursday, December 04, 2003 Description Transient Expression Assay Using Arabidopsis Mesophyll Protoplasts : PEG Transfection Procedure All steps are carried out at 23oC Add 20 ml DNA (20-40 mg of plasmid DNA of 5 kb in size) Add 200 ml pr...
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[Cell Culture] Transient Expression Assay Using Maize Mesophyll Protoplasts 日期：2006-07-24 14:10:54 点击：36 好评：0
Thursday, December 04, 2003 Description Transient Expression Assay Using Maize Mesophyll Protoplasts Procedure Plant Growth Soak seeds in water for overnight. Use half vermiculite and half pelite moss and germinate seeds under light for 3...
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[Cell Culture] Histochemical GUS assay 日期：2006-07-24 14:10:53 点击：51 好评：0
Thursday, December 04, 2003 Description The histochemical assay to screen for expression of b-glucurodinase (gus) activity in genetically modified plants is carried out according to the method of Jefferson et al. (1987) Procedure For analy...
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[Cell Culture] Cysteine proteinase activity assay 日期：2006-07-24 14:10:53 点击：19 好评：0
Thursday, December 04, 2003 Description The specific cysteine proteinase substrate Z-Phe-Arg-MCA is used as described by Barret and Kirschke (1981). The substrate, Z-Phe-Arg-NMec, which is barely fluorescent, is hydrolyzed to liberate 7-am...
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[Cell Culture] Western blot 日期：2006-07-24 14:10:52 点击：35 好评：0
Thursday, December 04, 2003 Description After extraction of soluble protein from leaves, samples are run on a SDS-PAGE gel. The method according to Sambrook et al. (1989) is used for SDS-PAGE and the method as described in the technical bo...
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[Cell Culture] Spermatozoa Chemotaxis assay using Zigmond Chamber. 日期：2006-07-24 14:10:52 点击：47 好评：0
Friday, December 19, 2003 Description This assay uses the zigmond chamber to study chemotaxis of spermatozoa or other migratory cells. The chamber consists of two wells separated by a bridge. The culture medium is placed on the bridge, cov...
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[Cell Culture] Determination of IC50 日期：2006-07-24 14:10:51 点击：21 好评：0
Saturday, January 17, 2004 Description In vitro whole cell assay by [H3] Hypoxanthine uptake assay Procedure [H3] Hypoxanthine uptake assay (with respect to red blood cell cultures of P. falciparum Dispense 25 ul complete RPMI in different...