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[Cell Culture] Protein Expression Screening Using Plant Tissue Prints On Ni 日期：2006-07-24 14:11:02 点击：36 好评：0
Friday, November 21, 2003 Description The first print of the stem will contain a lot of chlorophyll but the second or third print should reveal the position of antigens. This method transfers soluble molecules of all sorts to the membrane....
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[Cell Culture] Isolation of Chloroplasts from Spinach Leaves 日期：2006-07-24 14:11:01 点击：22 好评：0
Friday, November 21, 2003 Description This protocol describes a simple procedure for isolating chloroplasts from spinach leaves. Procedure 1. Prepare an ice bath and pre-cool all glassware to be used (including a mortar and pestle). 2. Sel...
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[Cell Culture] Lyophilizing Cells 日期：2006-07-24 14:11:01 点击：27 好评：0
Friday, November 21, 2003 Description Protocol for Lyophilization of bacteria Procedure Inoculate 200 ml L-broth supplemented with appropriate antibiotics with the bacteria to be lyophilized. Incubate the culture at 37癈 with vigorous shak...
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[Cell Culture] Giemsa for blood cells and parasites 日期：2006-07-24 14:11:00 点击：31 好评：0
Monday, November 24, 2003 Description Staining protocol Procedure For smears or cytospin preparations: Fix prepared film in methanol - 3 to 5 minutes. Stain in a mixture of 1ml of Giemsa stock plus 2mls of pH6.4 buffer and 47mls of distill...
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[Cell Culture] Leishmans stain for blood cells and parasites 日期：2006-07-24 14:11:00 点击：36 好评：0
Monday, November 24, 2003 Description Staining protocol Procedure Prepare cytospin slide or smear of sample. Either spray the wet preparation with Smear Fix? leave for 1-2 mins, wash off the fixative with distilled water and drain.... Or l...
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[Cell Culture] Acridine Orange Stain 日期：2006-07-24 14:10:59 点击：41 好评：0
Monday, November 24, 2003 Description Acridine orange is a fluorescent dye which will bind to the nucleic acid of bacteria and other cells. It is recommended for use for the detection of microorganisms in direct smears. It is useful for th...
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[Cell Culture] Gentle lysis of cells in sucrose-containing Mito-Buffer 日期：2006-07-24 14:10:59 点击：28 好评：0
Monday, November 24, 2003 Description Lysis in the sucrose-containing Buffer (Mito-Buffer) is supposed to prevent accidential disrupture of the mitochondria to prevent the leakage of mitochondrial proteins (such as cytochrome c) into the c...
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[Cell Culture] Culture of Human Prostatic Carcinoma Cell Lines 日期：2006-07-24 14:10:58 点击：27 好评：0
Monday, November 24, 2003 Description Culture of Human Prostatic Carcinoma Cell Lines Procedure Growing and splitting the cells The human prostatic carcinoma cell lines PC3, DU145, JCA1, ALVA31, and LNCaP can be cultured in standard RPMI c...
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[Cell Culture] ARABIDOPSIS ROOT TRANSFORMATION ASSAYS 日期：2006-07-24 14:10:57 点击：56 好评：0
Thursday, December 04, 2003 Description ARABIDOPSIS ROOT TRANSFORMATION ASSAYS Procedure SEED STERILIZATION 1. Sterilize Arabidopsis seeds for 10 min in a solution of 50% bleach plus 0.1% SDS. 2. Rinse 5 times with sterile dH2O. 3. Place 5...
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[Cell Culture] Agrobacterium-mediated Tobacco Transformation 日期：2006-07-24 14:10:56 点击：44 好评：0
Thursday, December 04, 2003 Description Agrobacterium-mediated Tobacco Transformation Procedure 1. Agrobacterium culture and Harvest: Agrobacterium overnight culture in YEP cfg 5000pm, washing in MS, dilution (1/10 of original volum) with...