Monday, May 10, 2004 Description The Following Protocol can be used to couple either FITC or Biotin to IgG. The same method is used for both, and when discrepancies occur, they will be noted. Procedure . Dissolve 2mg of IgG in 1mL of PBS (...

Monday, May 10, 2004 Description This protocols allows the use of fluorescence microscopy to visualize epitope-tagged receptors expressed by stable adherent cell lines. In contrast to the permeabilized cell protocol, this protocol where an...

Monday, May 10, 2004 Description This protocol will visualize both internalized receptors that were once on the plasma membrane and receptors in the biosynthetic pathway. This protocol was specifically written for visualizing agonist-induc...

Monday, May 10, 2004 Description Yeast IF with Methanol/Acetone Dehydration Procedure Grow cells at the appropriate temperature to 5x10E6 in 5 mls YPD. Add 0.5mls 37% formaldehyde (best grade) and incubate on the roller at same temp. for 1...

Monday, May 10, 2004 Description Yeast IF without Dehydration Procedure Grow cells at the appropriate temperature to 5x10E6 in 5 mls YPD. Add 0.5mls 37% formaldehyde (best grade) and incubate on the roller at same temp. for 10 min. Spin do...

Monday, May 10, 2004 Description RESPONDER CELL FREQUENCY ASSAY (RCF): LPA ?Limiting Dilution Procedure 1 This assay measures the frequency of pathogen-specific memory CD4 cells (1) by adding a limiting dilution step to a lymphocyte prolif...

Monday, May 10, 2004 Description WHOLE BLOOD LYMPHOCYTE PROLIFERATION ASSAY Procedure PRINCIPAL AND/OR PURPOSE OF THE TEST Lymphocyte proliferation assays (LPA) have been widely used to help describe the abnormalities associated with diver...

Monday, May 10, 2004 Description Toll-like receptor 4 (TLR4) and its associated MD-2 play a role in the response of myeloid cells to LPS. While the exact mechanism is not clearly defined at the present time, antibodies to TLR4 have been re...

Monday, May 10, 2004 Description Anti-TLR4/MD2 Neutralization of Lipid A-induced Cytokine Production by Mouse Peritoneal Cells Procedure Prepare peritoneal cells and dispense 1ml at 2x106/ml into each well of a 24-well culture plate. Pre-i...

Monday, May 10, 2004 Description Prior to using lymphoid tissue cell suspensions for flow cytometric analysis and/or for in vitro functional assays, it is recommended to remove red blood cells. The eBioscience 1X RBC Lysis Buffer (Cat. No....