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[Enzymology] Quick Virus/Viron Purification Protocol 日期：2005-07-18 00:00:00 点击：187 好评：0
Wednesday, October 29, 2003 Description This is a quick method for purifying virus from cell culture without the added hassle of sucrose gradients and identifying fractions positive for virus. This protocol is primarily designed for protei...
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[Enzymology] FAK Autophosphorylation Assay 日期：2005-07-18 00:00:00 点击：525 好评：0
Description This kinase assay is meant to determine whether an agonist or event can influence the autophosphorylation of FAK. The addition of 1 l of polyGT to the kinase reaction mix will determine the activity of the enzyme against a subs...
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[Enzymology] Histone H1 Kinase Activity Assay 日期：2005-07-18 00:00:00 点击：242 好评：0
Monday, October 27, 2003 Description This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1...
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[Enzymology] In-Gel Kinase Assay 日期：2005-07-18 00:00:00 点击：789 好评：25
Monday, October 27, 2003 Description This protocol describes how to assay for kinase activity within a polyacrylamide gel, rather than in solution. The advantages to an in-gel assay are that an apparent molecular weight can be assigned to...
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[Enzymology] Polymerase III In Vitro Transcription 日期：2005-07-18 00:00:00 点击：191 好评：0
Description This protocol describes an RNA Polymerase III (Pol III) transcription assay using an extract or proteins of choice. Pol III is the polymerase responsible for transcribing 5S RNA, tRNAs, and other small RNAs. á-Amanitin inhibit...
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[Enzymology] In Vitro Transcription Assay (Run-off&nb 日期：2005-07-18 00:00:00 点击：163 好评：0
Description With this protocol, transcripts that were initiated from specific genes by RNA polymerases prior to permeabilization can be measured. Instead of a nuclear extract, permeabilized cells are used. Procedure A. Permeabilization of...
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[Enzymology] Determination of the Molecular Weight of a DNA Binding Prote 日期：2005-07-18 00:00:00 点击：179 好评：0
Sunday, October 19, 2003 Description This protocol describes a procedure for determining the molecular weight of a DNA-binding protein. First, DNA containing a binding site (or multiple sites) for the protein of interest is labeled with [3...
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[Enzymology] Chloramphenicol Acetyl Transferase (CAT) Assay using n-Butry 日期：2005-07-18 00:00:00 点击：217 好评：0
Saturday, October 18, 2003 Description CAT activity can be used as a reporter to measure transcription. The CAT gene is inserted downstream from a promoter of interest and this construct is introduced into cells for expression. Extracts ar...
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[Enzymology] The Under-Agarose Migration Assay 日期：2005-07-18 00:00:00 点击：139 好评：0
Description The Under-Agarose assay is a useful method for observing the response of a cell population to one or more chemoattractant sources. The behavior of individual migrating cells can be studied by modifying the assay for video micro...
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[Enzymology] Measurement of Cell Adhesion Under Static Conditions 日期：2005-07-18 00:00:00 点击：105 好评：0
Saturday, October 18, 2003 Description Many different molecules have been described to promote cell adhesion including several cell surface carbohydrate-binding proteins. Measuring cell adhesion in the convenient 96-well microtiter plate f...