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[Enzymology] Tritiated thymidiene Uptake assay and MTT assay 日期：2005-07-18 00:00:00 点击：247 好评：-2
Saturday, October 18, 2003 Description Tritiated thymidiene Uptake assay and MTT assay To study if modulation of apoptosis by CD40 ligation and TNFa activation is by induction of proliferation or inhibition of apoptosis, following assays w...
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[Enzymology] Annexin V Staining Protocol 日期：2005-07-18 00:00:00 点击：331 好评：0
Friday, October 17, 2003 Description Annexin V Staining Protocol Procedure 1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of ~1 x 10 6 cells/ml. 2. Transfer 100 l of the solution (~1 x 10...
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[Enzymology] Radioiodination of cell surface proteins 日期：2005-07-18 00:00:00 点击：167 好评：0
Friday, October 17, 2003 Description Radioiodination of cell surface proteins Procedure 1. Wash cells at least three times with PBS and resuspend in 1 ml of PBS cntg. glucose at 0.5 - 2 x 107 cells/ml) in 15 ml conical tube, hold on ice 2....
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[Enzymology] Radioiodination of protein in solution 日期：2005-07-18 00:00:00 点击：187 好评：0
Friday, October 17, 2003 Description Addition of oxidizing reagents (such as chloramine-T or peroxidase+H202) converts I- to I+ or I3-. This highly reactive molecule attacks o-position of tyrosine (or in some case, histidine is also labele...
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[Enzymology] In vitro Sphingomyelinase Assay 日期：2005-07-18 00:00:00 点击：235 好评：0
Friday, October 17, 2003 Description In vitro Sphingomyelinase Assay Procedure Isolating cellular enzyme 1) Grow 5 x 108 cells under regular growth conditions. 2) Pellet cells and wash one time with ice cold PBS. 3) Resuspend pellet in 10...
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[Enzymology] Conjugation of Antibody to HRP 日期：2005-07-18 00:00:00 点击：170 好评：0
Friday, October 17, 2003 Description Conjugation of Antibody to HRP Procedure A. Preparation of Thiolated Antibody 1. Concentrate the antibody to 5-7 mg/ml 2. Dissolve 10 mg of SATA in 1 ml DMF (SATA:DMF) 3. Add 3.0 ml SATA:DMF per mg of a...
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[Enzymology] Kinase end-labeling of DNA 日期：2005-07-18 00:00:00 点击：168 好评：0
Wednesday, October 08, 2003 Description Typical 5'-kinase labeling reactions included the DNA to be labeled, [[gamma]]-32-P-dATP, T4 polynucleotide kinase, and buffer (1). After incubation at 37degC, reactions are heat inactivated by incub...
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[DNA Library] Easy 4 degree competent cells 日期：2005-07-18 00:00:00 点击：304 好评：0
Thursday, November 17, 2005 Description A method for preparing competent E. coli for transformation. The key advantage is that these cells can be kept for a week at 4 degrees Celsius. Procedure 1. Grow an overnight culture of E. coli XL-1...
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[DNA Library] Calcium chloride Competent cells Preparation and transformat 日期：2005-07-18 00:00:00 点击：599 好评：0
Tuesday, November 02, 2004 Description This is one of the standard protocols to prepare competent cells. In this the cells at a particular density are washed with CaCl2 which later helps in the transformation of plasmids into these cells....
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[DNA Library] PAH AluI Polymorphism 日期：2005-07-18 00:00:00 点击：310 好评：0
Saturday, May 15, 2004 Description PAH AluI Polymorphism Procedure AluI; LOCATED IN EXON 7 Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. PAHALU333 5' CTTGCACTGGTTTCCGCCTC 3' 100 ng/reaction Primer 2. PAHALU237 5' CCCAA...