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[General Lab] Pollen Development in Anthers of Arabidopsis 日期：2005-07-18 00:00:00 点击：163 好评：0
Friday, November 07, 2003 Description Pollen Development in Anthers of Arabidopsis Procedure . Sow Arabidopsis seeds in Petri dishes on a layer of sterilized superfine grade Perlite pre-wetted with Gamborg's B5 Medium. 2. Incubate in a gro...
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[General Lab] ARABIDOPSIS TRANSFORMATION 日期：2005-07-18 00:00:00 点击：154 好评：0
Friday, November 07, 2003 Description ARABIDOPSIS TRANSFORMATION Procedure Seed Sterilization. Sterilize WS (Wassilewskija) seed for 10 min in a solution of 50% Chlorox with 0.1% SDS in ependorf tubes. Rinse 3 to 5 times with dH20. Dry tho...
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[General Lab] Shortcut Yeast transformation 日期：2005-07-18 00:00:00 点击：212 好评：0
Friday, October 24, 2003 Description Method to transform plasmid DNA into yeast using a small amount of at-the-bench time. Requires 1 overnight to complete. Procedure 1. Take fresh overnight of yeast cells from liquid or media rich plate....
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[General Lab] Media for the Growth of Bactertial Symbionts for Entomopatho 日期：2005-07-18 00:00:00 点击：203 好评：0
Friday, October 24, 2003 Description Exact preparation of plates with lawns of bacteria for the in vitro growth of entomopahtogenic nematodes belong to Heterorhabditis and Steinernema Procedure Here's the protocol for raising nematodes on...
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[General Lab] Gel Shift with Glutaraldehyde 日期：2005-07-18 00:00:00 点击：162 好评：0
Sunday, October 19, 2003 Description Gel Shift with Glutaraldehyde Procedure 1. Pour a 4% acrylamide high ionic strength Tris/Glycine gel that is 1.5 mm thick with a 12 to 14 tooth comb. Combine: 6.7 ml 30 % Acrylamide (60:1), 10 ml 5X Tri...
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[General Lab] Ultraviolet (UV) Photochemical Crosslinking to Detect RNA Bi 日期：2005-07-18 00:00:00 点击：154 好评：0
Sunday, October 19, 2003 Description Labeled nucleotides from the RNA are covalently transferred to bound proteins following illumination by UV light. Excess RNA is then digested with Ribonuclease A and the labeled proteins are visualized...
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[General Lab] Bioassay TNF-alpha 日期：2005-07-18 00:00:00 点击：155 好评：0
Saturday, October 18, 2003 Description TNF-alpha Bioassay Procedure 1) Prepare L929 cell suspension at a density of 3.5 x 105/ml in assay medium. Add 100 l/well to the 96-well Assay Plate and incubate overnight at 37 degrees C, 5% CO2 in a...
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[General Lab] Dynamic Flow Assay in a Parallel Plate Flow Chamber 日期：2005-07-18 00:00:00 点击：139 好评：0
Saturday, October 18, 2003 Description Flow assays allow visualization of cell adhesion under well-defined wall shear stress. The visualization of the different events of cell adhesion can be quantified by selective image acquisition and s...
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[General Lab] Fluorescein labeling of proteins 日期：2005-07-18 00:00:00 点击：152 好评：0
Saturday, October 18, 2003 Description This method uses carboxyfluorescein succinimidyl ester (CFSE) rather than fluorescein isothiocyanate, resulting in more reliable labeling. Succinimidyl esters are excellent reagents for amine modifica...
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[General Lab] TRANSFECTION OF MAMMALIAN CELLS USING LIPOFECTAMINE REAGENT 日期：2005-07-18 00:00:00 点击：193 好评：0
Thursday, October 16, 2003 Description TRANSFECTION OF MAMMALIAN CELLS USING LIPOFECTAMINE REAGENT Procedure 1. In a six-well or 35 mm tissue culture plate, seed ~2x 105 cells per well in 2 ml Basal Medium containing 10% FBS. 2. Incubate t...