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[General Lab] Rhodamine-Phalloidin/Calcofluor Staining 日期：2005-07-18 00:00:00 点击：157 好评：0
Tuesday, November 18, 2003 Description Rhodamine-Phalloidin/Calcofluor Staining Procedure Grow 50 mls of yeast to 5x10E6. Add formadehyde to the media to 4% (33 mls. of 10%). Fix in media at temperature for 10 min. Spin down cells 2-3 K fo...
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[General Lab] Rhodamine-Phalloidin/Calcofluor Staining 日期：2005-07-18 00:00:00 点击：216 好评：0
Tuesday, November 18, 2003 Description Rhodamine-Phalloidin/Calcofluor Staining Procedure Grow 50 mls of yeast to 5x10E6. Add formadehyde to the media to 4% (33 mls. of 10%). Fix in media at temperature for 10 min. Spin down cells 2-3 K fo...
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[General Lab] Yeast Cell Cycle by Flow Cytometry 日期：2005-07-18 00:00:00 点击：181 好评：0
Tuesday, November 18, 2003 Description Yeast Cell Cycle by Flow Cytometry Procedure Spin down 107 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store...
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[General Lab] Yeast IF without Dehydration 日期：2005-07-18 00:00:00 点击：126 好评：0
Tuesday, November 18, 2003 Description Yeast IF without Dehydration Procedure Grow cells at the appropriate temperature to 5x10E6 in 5 mls YPD. Add 0.5mls 37% formaldehyde (best grade) and incubate on the roller at same temp. for 10 min. S...
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[General Lab] Yeast IF with Methanol/Acetone Dehydration 日期：2005-07-18 00:00:00 点击：178 好评：0
Tuesday, November 18, 2003 Description Yeast IF with Methanol/Acetone Dehydration Procedure Grow cells at the appropriate temperature to 5x10E6 in 5 mls YPD. Add 0.5mls 37% formaldehyde (best grade) and incubate on the roller at same temp....
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[General Lab] Quantitative Mating Assay 日期：2005-07-18 00:00:00 点击：118 好评：0
Tuesday, November 18, 2003 Description Quantitative Mating Assay (Yeast) Procedure 1. Grow a culture of the strain whose mating efficiency is to be determined to a density of about 1E7 cells/ml in YEPD. Prepare a similar culture of an appr...
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[General Lab] Yeast Vivisection 日期：2005-07-18 00:00:00 点击：183 好评：0
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[General Lab] Sporulation Protocol 2 日期：2005-07-18 00:00:00 点击：195 好评：0
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[General Lab] Sporulation Protocol 日期：2005-07-18 00:00:00 点击：180 好评：0
Tuesday, November 18, 2003 Description One of the suggested protocol Procedure Grow up a small (5mL) overnight culture in YEPD (rich glucose media), 30C. Count the cells the next day, and dilute the culture down to 1E6 cells/mL, again into...
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[General Lab] BAC DNA Fingerprint Analysis 日期：2005-07-18 00:00:00 点击：304 好评：0
Monday, November 10, 2003 Description BAC DNA Fingerprint Analysis Procedure Fingerprinting Protocol (Agarose Gel): Restriction digests consist of: 15.75 ml ddH2O 1 l 10 X buffer B (Boehringer Mannheim) 0.25 l HindIII (40 U/l) 3 l DNA Set...