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  • [PCR] Disruption by Fusion PCR 日期:2005-07-18 00:00:00 点击:289 好评:0

    Tuesday, November 18, 2003 Description Disruption by Fusion PCR Procedure 1) In separate PCR reactions, amplify the 5' and 3' ends of the gene of interest with primers about 200 bases apart. Primer 2 should begin with 24 nts complementary...

  • [PCR] UV Mutagenesis 日期:2005-07-18 00:00:00 点击:328 好评:0

    Tuesday, November 18, 2003 Description UV Mutagenesis Procedure 1) Grow cells 2-3 days to stationary phase 2) Wash cells 2x with water 3) Count cells (vortex well to separate cells as much as possible) 4) Resuspend to 2 x 108 cells/ml 5) T...

  • [PCR] Vectorette PCR of Yeast DNA 日期:2005-07-18 00:00:00 点击:248 好评:0

    Tuesday, November 18, 2003 Description Vectorette PCR of Yeast DNA Procedure 1) Cut 1-3 g of clean DNA overnight with 8-10U of blunt cutting enzyme in 20l Most problems come from dirty, uncut DNA. Phenol/glass bead/RNase prepared DNA works...

  • [PCR] Yeast Colony PCR 日期:2005-07-18 00:00:00 点击:312 好评:0

    Tuesday, November 18, 2003 Description Yeast Colony PCR Procedure PCR mixture Combine reaction mix on ice: 2.5 μl 10X Colony PCR Buffer 1.5 μl 25 mM MgCl2 0.5 μl 10 mM dNTP's 10 pmols of each primer 1.25 μl 20% Triton X-100 0.25 μl Ta...

  • [PCR] Leaf GUS Assay 日期:2005-07-18 00:00:00 点击:229 好评:0

    Monday, November 10, 2003 Description Leaf GUS Assay Procedure This protocol is for small samples (usually single leaf from 21DAI plants), scale up for larger samples As there are usually many samples, we use a microtitre plate and plate r...

  • [PCR] Inverse PCR Protocol 日期:2005-07-18 00:00:00 点击:421 好评:0

    Monday, November 10, 2003 Description Inverse PCR Protocol This method can be used directly for pBIN19 or pBI101 based T-DNA inserts Procedure Digestion reaction: 2.5g genomic DNA (CTAB) 1x 10x enzyme buffer 20-40U restriction enzyme xl dd...

  • [PCR] Competitive Ligand Binding to Cortical Receptor using Crossl 日期:2005-07-18 00:00:00 点击:297 好评:0

    Sunday, October 19, 2003 Description This protocol describes a competitive ligand binding assay for cortical neurotrophin receptors. Following binding in the presence of competitor, the bound radiolabeled ligand is cross-linked to the rece...

  • [PCR] Gel Mobility Shift Assay Using Magnesium Chloride and EDTA 日期:2005-07-18 00:00:00 点击:324 好评:0

    Sunday, October 19, 2003 Description Gel Mobility Shift Assay Using Magnesium Chloride and EDTA Procedure A. Gel Shift Reaction 1. Prepare a 20 l binding reaction by combining the following ingredients: 4 l of 5X Binding Buffer 0.2 l of 0....

  • [PCR] Beta-Lactamase Induction (Crude Preparation) 日期:2005-07-18 00:00:00 点击:269 好评:0

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  • [Immunology] ISOLATION AND CULTURE OF MOUSE BONE MARROW-DERIVED MACROPHAG 日期:2005-07-18 00:00:00 点击:340 好评:0

    Wednesday, July 27, 2005 Description Principle: Mononuclear phagocyte progenitor cells derived from femoral and tibial bone marrow are propagated in the presence of M-CSF. This macrophage growth factor is secreted by L929 cells and is used...

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