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[Cell Culture] Antigen Retrieval 日期：2006-07-24 14:10:45 点击：90 好评：0
Monday, May 03, 2004 Description Some other useful protocols can be found in the following references: Caltuvetti et. al., 1993, J. Pathology 171:83 and Shi et. al., 1991 J. Hist. Cytochem. 39:741. Procedure Place the slides in a plastic c...
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[Cell Culture] PEROXIDASE-ANTI-PEROXIDASE TECHNIQUE FOR THE IDENTIFICATION 日期：2006-07-24 14:10:44 点击：29 好评：0
Monday, May 03, 2004 Description PEROXIDASE-ANTI-PEROXIDASE TECHNIQUE FOR THE IDENTIFICATION OF ANTIGENS IN PARAFFIN WAX SECTIONS Procedure Dewax sections and transfer to 100% alcohol. Block endogenous peroxidase with 0.5% hydrogen peroxid...
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[Cell Culture] Cytokine-Induced Proliferation of Indicator Cell Lines 日期：2006-07-24 14:10:44 点击：30 好评：0
Monday, May 03, 2004 Description Cytokine-Induced Proliferation of Indicator Cell Lines Procedure Materials Indicator cell line (see Quick Guide Chart for a given cytokine) Culture Medium (RPMI-1640 supplemented with 10% FBS) 96-well flat-...
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[Cell Culture] TNF-alpha-Induced Killing of L929 Cell Line 日期：2006-07-24 14:10:43 点击：77 好评：0
Monday, May 03, 2004 Description TNF-alpha-Induced Killing of L929 Cell Line Procedure Materials L929 mouse fibroblast line (ATCC Cat. No. CCL-1) Culture Medium (RPMI supplemented with 10% FBS) Assay Medium (RPMI supplemented with 2% FBS)...
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[Cell Culture] IFN-gamma Protection from Viral Infection of Cell Lines 日期：2006-07-24 14:10:43 点击：27 好评：0
Monday, May 03, 2004 Description IFN-gamma Protection from Viral Infection of Cell Lines Procedure Materials L929 mouse fibroblast line (ATCC Cat. No. CCL-1) or A549 human lung carcinoma (ATCC Cat. No. CCL-185) Culture Medium (RPMI supplem...
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[Cell Culture] Cytokine ELISPOT Protocol 日期：2006-07-24 14:10:42 点击：50 好评：0
Monday, May 03, 2004 Description ELISPOT (Enzyme-linked ImmunoSPOT) technique was originally used to enumerate antibody secreting B cells. In the current technique, cells are deposited onto a membrane coated with one antibody specific for...
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[Cell Culture] Antibody Neutralization of Cytokine-induced Proliferation of 日期：2006-07-24 14:10:42 点击：22 好评：0
Monday, May 03, 2004 Description Antibody Neutralization of Cytokine-induced Proliferation of Indicator Cell Lines Procedure Materials Indicator cell line (see Quick Guide Chart for a given cytokine) Culture Medium (RPMI supplemented with...
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[Cell Culture] Antibody Neutralization of TNFa-Induced Killing of L929 Cell 日期：2006-07-24 14:10:41 点击：34 好评：0
Monday, May 03, 2004 Description Antibody Neutralization of TNFa-Induced Killing of L929 Cell Line Procedure Materials L929 mouse fibroblast line (ATCC Cat. No. CCL-1) Culture Medium (RPMI supplemented with 10% FBS) Assay Medium (RPMI supp...
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[Cell Culture] Antibody Neutralization of IFNg-Protection from Viral Infect 日期：2006-07-24 14:10:41 点击：20 好评：0
Monday, May 03, 2004 Description Antibody Neutralization of IFNg-Protection from Viral Infection of L929 and A549 Cell Lines Procedure Materials L929 mouse fibroblast line (ATCC Cat. No. CCL-1) or A549 human lung carcinoma (ATCC Cat. No. C...
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[Cell Culture] Pediatric Virus-specific Bulk Stimulation Cytotoxic T Lympho 日期：2006-07-24 14:10:40 点击：26 好评：0
Wednesday, May 05, 2004 Description This protocol is designed to increase the sensitivity of detection of HIV-1 specific CTL by the antigen-stimulation of PBMC. Stimulator cells are prepared by infecting autologous B-lymphoblastoid cell li...