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[Cell Culture] HUMAN SOLUBLE INTERLEUKIN-2 RECEPTOR 日期：2006-07-24 14:10:39 点击：60 好评：0
Wednesday, May 05, 2004 Description IL-2 receptor alpha chain is one of the key components of the lymphoid cell receptor complex. Interleukin-2(IL-2), plays a pivotal role in initiating and potentiating the immune response. It provides a c...
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[Cell Culture] DETECTION OF ISOTYPE SPECIFIC IgG ANTIBODIES 日期：2006-07-24 14:10:39 点击：40 好评：0
Wednesday, May 05, 2004 Description Response to immunization with Tetanus Toxoid Vaccine is an indicator of immune function in infants and children which can be measured by comparing the amount of specific antibody detected before and afte...
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[Cell Culture] ELISA, Antibody capture 日期：2006-07-24 14:10:38 点击：39 好评：0
Wednesday, May 05, 2004 Description ELISA, Antibody capture Procedure 1. Dilute protein in PBS (2.5-5 mg/ml), add 100 ml/well. Incubate overnight at 4?C or at room temperature for 1 h. 2. Block plates with 250 ml PBS/1% BSA for overnight a...
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[Cell Culture] Sandwich ELISA for SCCA1 and SCCA2 日期：2006-07-24 14:10:38 点击：33 好评：0
Wednesday, May 05, 2004 Description Sandwich ELISA for SCCA1 and SCCA2 Procedure 1. Dilute affinity purified rabbit anti-SCCA 1:400 in PBS (appropriate dilution of this antibody needs to be determined following each batch affinity purifica...
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[Cell Culture] Immunofluorescence Yeast 日期：2006-07-24 14:10:35 点击：32 好评：0
Wednesday, May 05, 2004 Description Immunofluorescence Yeast Procedure 1) Grow cells in YEPD or defined media to a concentration of 1-5 X107(protocol is for ~5 ml of exponentially growing cells). 2) Add 0.6 ml of 37% formaldehyde solution...
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[Cell Culture] Iindirect immunoflourescence for yeast: 日期：2006-07-24 14:10:35 点击：33 好评：0
Monday, May 10, 2004 Description Iindirect immunoflourescence for yeast: Procedure 1mL yeast (~0.2-0.5 OD) + 0.1mL 36% formaldehyde, 60'/Rt Wash 2x 1mL H2O (spin 10K, 5). Wash in 1mL SK and resuspend in 450 ul SK. Sonicate 5 sec. lowest se...
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[Cell Culture] IIF Protocol (modifications for maintaining GFP fluorescence 日期：2006-07-24 14:10:34 点击：43 好评：0
Monday, May 10, 2004 Description IIF Protocol (modifications for maintaining GFP fluorescence Procedure Grow cells to OD 0.5 Harvest 0.5-1 ml cells. Resuspend in PBS, and add PFA to final concentration of 4%. If using the regular formaldeh...
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[Cell Culture] PDS1::HA IMMUNOFLUORESCENCE 日期：2006-07-24 14:10:33 点击：54 好评：0
Monday, May 10, 2004 Description PDS1::HA IMMUNOFLUORESCENCE Procedure Cultures: 1500, 1501 Fixations: 10, 20, 30 and 45 minutes in 4% formaldehyde, 0.1 M KPO4 pH 6.5; 15 minutes in 4% formaldehyde, 50 mM KPO4 pH 6.5, 0.5 mM MgCl2 Grow cel...
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[Cell Culture] Antibody Conjugation 日期：2006-07-24 14:10:33 点击：39 好评：0
Monday, May 10, 2004 Description The Following Protocol can be used to couple either FITC or Biotin to IgG. The same method is used for both, and when discrepancies occur, they will be noted. Procedure . Dissolve 2mg of IgG in 1mL of PBS (...
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[Cell Culture] Staining for Surface Receptors 日期：2006-07-24 14:10:32 点击：26 好评：0
Monday, May 10, 2004 Description This protocols allows the use of fluorescence microscopy to visualize epitope-tagged receptors expressed by stable adherent cell lines. In contrast to the permeabilized cell protocol, this protocol where an...