Saturday, May 15, 2004 Description HOXB3 TaqI Polymorphism Procedure TaqI in HOXB3; Clone pH 2-1-5 Template: Genomic DNA 100-200 ng/reaction volume of 25 祃 Primer 1. H2GCK3 '5-TAT TTT TGT GAT CAA TGG AGA CC 100 ng/reaction Primer 2. H2GCK...
Saturday, May 15, 2004 Description PAH MspI Polymorphism Procedure MspI; LOCATED NEAR THE 5' END OF INTRON 8 Template: Genomic DNA 100-200 ng/reaction volume of 25 祃 Primer 1. PAHM 5 -GAA GCA TAT TGT ATC TGC CC -3' 100 ng/reaction Primer...
Saturday, May 15, 2004 Description RET TaqI Polymorphism Procedure Taq I polymorphism located in Exon 13 Template: Genomic DNA 50-100 ng/reaction volume of 25 ul Primer 1. RETEX13F: 5'-CTC TCT GTC TGA ACT TGG GC-3' 60 ng/Reaction Primer 2....
Saturday, May 15, 2004 Description PAH BglII Polymorphism Procedure BglII; LOCATED 5' OF EXON 1 Template: Genomic DNA 100-200 ng/reaction volume of 25 祃 Primer 1. B2PAH1 5' -GCA GGA AAC TCT CTG ACT TTG G -3' 100 ng/reaction Primer 2. B2PA...
Saturday, May 15, 2004 Description COMT BglI Polymorphism Procedure Bgl I (modified from Chen et al., 1996) Template: Genomic DNA 100-200 ng/reaction volume of 25 祃. Primer 1. COMTBGL1F 5'--TGC GGA AGG GGA CAG TGC TAC--3' 100 ng/Reaction...
Saturday, May 15, 2004 Description APOB XbaI Polymorphism Procedure Xba I Template: Genomic DNA 100-200 ng/reaction volume of 25 祃 Primer 1. APOBXB1: 5'-AAA TAA CCT TAA TCA TCA ATT GGT 3' 100ng/reaction Primer 2. APOBXB2: 5'-GGT TCT TAG C...
Saturday, May 15, 2004 Description PAH XmnI Polymorphism Procedure XmnI; LOCATED ABOUT 2 KB 3' OF THE MSPI POLYMORPHISM IN INTRON 8 Template: Genomic DNA 100-200 ng/reaction volume of 25 祃 Primer 1. PAHXMN1 5 -CTG TAC TTG TAA GAT GCA GC-3...
Monday, June 07, 2004 Description This protocol describes a method that can be used to clone individual cells. This is done by limiting dilution and examination of plates for single cells. Growth factors or selection components may be adde...
Tuesday, June 29, 2004 Description This protocol describes how to freeze tissue culture cells (mamalian) in a way that they will be viable when thawed. Procedure 1.) Aspirate media 2.) Wash cells with PBS 3.) Apply trypsin/EDTA (1ml for 15...
Saturday, April 30, 2005 Description A method to prepare large ammounts or synthetic RNA using T7, T3 or SP6 RNA polymerases. Procedure Template Preparation and RNA Transcription PCR generated templates or 2 x Cesium Cl2 banded plasmid DNA...