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[Cell Culture] Staining for total receptors 日期：2006-07-24 14:10:32 点击：23 好评：0
Monday, May 10, 2004 Description This protocol will visualize both internalized receptors that were once on the plasma membrane and receptors in the biosynthetic pathway. This protocol was specifically written for visualizing agonist-induc...
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[Cell Culture] Yeast IF with Methanol/Acetone Dehydration 日期：2006-07-24 14:10:31 点击：32 好评：0
Monday, May 10, 2004 Description Yeast IF with Methanol/Acetone Dehydration Procedure Grow cells at the appropriate temperature to 5x10E6 in 5 mls YPD. Add 0.5mls 37% formaldehyde (best grade) and incubate on the roller at same temp. for 1...
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[Cell Culture] Yeast IF without Dehydration 日期：2006-07-24 14:10:31 点击：41 好评：0
Monday, May 10, 2004 Description Yeast IF without Dehydration Procedure Grow cells at the appropriate temperature to 5x10E6 in 5 mls YPD. Add 0.5mls 37% formaldehyde (best grade) and incubate on the roller at same temp. for 10 min. Spin do...
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[Cell Culture] RESPONDER CELL FREQUENCY ASSAY (RCF): LPA ?Limiting Dilution 日期：2006-07-24 14:10:30 点击：66 好评：0
Monday, May 10, 2004 Description RESPONDER CELL FREQUENCY ASSAY (RCF): LPA ?Limiting Dilution Procedure 1 This assay measures the frequency of pathogen-specific memory CD4 cells (1) by adding a limiting dilution step to a lymphocyte prolif...
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[Cell Culture] WHOLE BLOOD LYMPHOCYTE PROLIFERATION ASSAY 日期：2006-07-24 14:10:30 点击：38 好评：0
Monday, May 10, 2004 Description WHOLE BLOOD LYMPHOCYTE PROLIFERATION ASSAY Procedure PRINCIPAL AND/OR PURPOSE OF THE TEST Lymphocyte proliferation assays (LPA) have been widely used to help describe the abnormalities associated with diver...
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[Cell Culture] Anti-TLR4 Neutralization of LPS-induced Cytokine Production 日期：2006-07-24 14:10:29 点击：34 好评：0
Monday, May 10, 2004 Description Toll-like receptor 4 (TLR4) and its associated MD-2 play a role in the response of myeloid cells to LPS. While the exact mechanism is not clearly defined at the present time, antibodies to TLR4 have been re...
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[Cell Culture] Anti-TLR4/MD2 Neutralization of Lipid A-induced Cytokine Pro 日期：2006-07-24 14:10:28 点击：35 好评：0
Monday, May 10, 2004 Description Anti-TLR4/MD2 Neutralization of Lipid A-induced Cytokine Production by Mouse Peritoneal Cells Procedure Prepare peritoneal cells and dispense 1ml at 2x106/ml into each well of a 24-well culture plate. Pre-i...
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[Cell Culture] Protocol for RBC Lysis of Mouse Spleen 日期：2006-07-24 14:10:28 点击：40 好评：0
Monday, May 10, 2004 Description Prior to using lymphoid tissue cell suspensions for flow cytometric analysis and/or for in vitro functional assays, it is recommended to remove red blood cells. The eBioscience 1X RBC Lysis Buffer (Cat. No....
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[Cell Culture] Metaphase chromosome preparation 日期：2006-07-24 14:10:27 点击：29 好评：0
Saturday, May 15, 2004 Description Metaphase chromosome preparation Procedure Incubate culture for 72 hours in CO2 cell culture incubator, mix flask 1-2 times per day Add Colcemid (about 45 min before harvesting) Make 2 aliquots and transf...
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[Cell Culture] ADH2 Intron 3 RsaI Polymorphism 日期：2006-07-24 14:10:27 点击：38 好评：0
Saturday, May 15, 2004 Description ADH2 Intron 3 RsaI Polymorphism Procedure TEMPLATE: Genomic DNA 100ng/reaction volume of 25 祃 PRIMER 1. A2IN3DW3 5' ATA TTT ATT TTA CCC TAA ACT TAT G 3' PRIMER 2. A2IN3UP2 5' GAG CTA AAA CAT ACT TTG GAT...