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[Cell Culture] Protein extraction for SDS-PAGE from human monocytes 日期：2006-07-24 14:10:19 点击：46 好评：0
Wednesday, May 18, 2005 Description Protocol for rapid extraction of undegraded proteins from cultured human monocytes for the purpose of immunoblotting Procedure Isolate PBMCs from Buffy Coats as usual, and select CD14+ monocytes using an...
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[Cell Culture] Primary neuron viability 日期：2006-07-24 14:10:18 点击：31 好评：0
Wednesday, June 01, 2005 Description Fluorescence based asssay for establishing neuronal viability in cell culture monolayers. Procedure Monolayer cultures of rat embryonic cortical neurons were prepared and plated at a density of 2-5 x 10...
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[Cell Culture] Dorsal Root Ganglion Neuron Culture from Adult Rats 日期：2006-07-24 14:10:17 点击：43 好评：0
Sunday, June 12, 2005 Description Procedure for culturing and maintaining Dorsal Root Ganglion (DRG) neurons from adult rats. Procedure Preparing Culture Surface -Collagen coat cover slips (if using) or cell culture dishes: evenly spread 2...
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[Cell Culture] Differentiation of 3T3-L1 fibroblasts to adipocytes 日期：2006-07-24 14:10:16 点击：36 好评：0
Tuesday, June 14, 2005 Description We maintain the stock of 3T3-L1 cells in 150 mm tissue culture dishes in DMEM (Gibco catalog no. 11995-065) supplemented with 10% Fetal Bovine Serum, 100 units/ml of Penicillin and 100 礸/mL of Streptomyc...
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