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[Cell Culture] Yeast IF with Methanol/Acetone Dehydration 日期：2006-07-24 14:10:31 点击：32 好评：0
Monday, May 10, 2004 Description Yeast IF with Methanol/Acetone Dehydration Procedure Grow cells at the appropriate temperature to 5x10E6 in 5 mls YPD. Add 0.5mls 37% formaldehyde (best grade) and incubate on the roller at same temp. for 1...
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[Cell Culture] Yeast IF without Dehydration 日期：2006-07-24 14:10:31 点击：41 好评：0
Monday, May 10, 2004 Description Yeast IF without Dehydration Procedure Grow cells at the appropriate temperature to 5x10E6 in 5 mls YPD. Add 0.5mls 37% formaldehyde (best grade) and incubate on the roller at same temp. for 10 min. Spin do...
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[Cell Culture] RESPONDER CELL FREQUENCY ASSAY (RCF): LPA ?Limiting Dilution 日期：2006-07-24 14:10:30 点击：69 好评：0
Monday, May 10, 2004 Description RESPONDER CELL FREQUENCY ASSAY (RCF): LPA ?Limiting Dilution Procedure 1 This assay measures the frequency of pathogen-specific memory CD4 cells (1) by adding a limiting dilution step to a lymphocyte prolif...
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[Cell Culture] WHOLE BLOOD LYMPHOCYTE PROLIFERATION ASSAY 日期：2006-07-24 14:10:30 点击：38 好评：0
Monday, May 10, 2004 Description WHOLE BLOOD LYMPHOCYTE PROLIFERATION ASSAY Procedure PRINCIPAL AND/OR PURPOSE OF THE TEST Lymphocyte proliferation assays (LPA) have been widely used to help describe the abnormalities associated with diver...
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[Cell Culture] Anti-TLR4 Neutralization of LPS-induced Cytokine Production 日期：2006-07-24 14:10:29 点击：37 好评：0
Monday, May 10, 2004 Description Toll-like receptor 4 (TLR4) and its associated MD-2 play a role in the response of myeloid cells to LPS. While the exact mechanism is not clearly defined at the present time, antibodies to TLR4 have been re...
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[Cell Culture] Anti-TLR4/MD2 Neutralization of Lipid A-induced Cytokine Pro 日期：2006-07-24 14:10:28 点击：35 好评：0
Monday, May 10, 2004 Description Anti-TLR4/MD2 Neutralization of Lipid A-induced Cytokine Production by Mouse Peritoneal Cells Procedure Prepare peritoneal cells and dispense 1ml at 2x106/ml into each well of a 24-well culture plate. Pre-i...
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[Cell Culture] Protocol for RBC Lysis of Mouse Spleen 日期：2006-07-24 14:10:28 点击：40 好评：0
Monday, May 10, 2004 Description Prior to using lymphoid tissue cell suspensions for flow cytometric analysis and/or for in vitro functional assays, it is recommended to remove red blood cells. The eBioscience 1X RBC Lysis Buffer (Cat. No....
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[Cell Culture] Metaphase chromosome preparation 日期：2006-07-24 14:10:27 点击：29 好评：0
Saturday, May 15, 2004 Description Metaphase chromosome preparation Procedure Incubate culture for 72 hours in CO2 cell culture incubator, mix flask 1-2 times per day Add Colcemid (about 45 min before harvesting) Make 2 aliquots and transf...
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[Cell Culture] ADH2 Intron 3 RsaI Polymorphism 日期：2006-07-24 14:10:27 点击：38 好评：0
Saturday, May 15, 2004 Description ADH2 Intron 3 RsaI Polymorphism Procedure TEMPLATE: Genomic DNA 100ng/reaction volume of 25 祃 PRIMER 1. A2IN3DW3 5' ATA TTT ATT TTA CCC TAA ACT TAT G 3' PRIMER 2. A2IN3UP2 5' GAG CTA AAA CAT ACT TTG GAT...
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[Cell Culture] HOXB3 TaqI Polymorphism 日期：2006-07-24 14:10:26 点击：38 好评：0
Saturday, May 15, 2004 Description HOXB3 TaqI Polymorphism Procedure TaqI in HOXB3; Clone pH 2-1-5 Template: Genomic DNA 100-200 ng/reaction volume of 25 祃 Primer 1. H2GCK3 '5-TAT TTT TGT GAT CAA TGG AGA CC 100 ng/reaction Primer 2. H2GCK...
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