Monday, November 24, 2003 Description Staining protocol Procedure For smears or cytospin preparations: Fix prepared film in methanol - 3 to 5 minutes. Stain in a mixture of 1ml of Giemsa stock plus 2mls of pH6.4 buffer and 47mls of distill...

Monday, November 24, 2003 Description Staining protocol Procedure Prepare cytospin slide or smear of sample. Either spray the wet preparation with Smear Fix? leave for 1-2 mins, wash off the fixative with distilled water and drain.... Or l...

Monday, November 24, 2003 Description Acridine orange is a fluorescent dye which will bind to the nucleic acid of bacteria and other cells. It is recommended for use for the detection of microorganisms in direct smears. It is useful for th...

Monday, November 24, 2003 Description Lysis in the sucrose-containing Buffer (Mito-Buffer) is supposed to prevent accidential disrupture of the mitochondria to prevent the leakage of mitochondrial proteins (such as cytochrome c) into the c...

Monday, November 24, 2003 Description Culture of Human Prostatic Carcinoma Cell Lines Procedure Growing and splitting the cells The human prostatic carcinoma cell lines PC3, DU145, JCA1, ALVA31, and LNCaP can be cultured in standard RPMI c...

Thursday, December 04, 2003 Description ARABIDOPSIS ROOT TRANSFORMATION ASSAYS Procedure SEED STERILIZATION 1. Sterilize Arabidopsis seeds for 10 min in a solution of 50% bleach plus 0.1% SDS. 2. Rinse 5 times with sterile dH2O. 3. Place 5...

Thursday, December 04, 2003 Description Agrobacterium-mediated Tobacco Transformation Procedure 1. Agrobacterium culture and Harvest: Agrobacterium overnight culture in YEP cfg 5000pm, washing in MS, dilution (1/10 of original volum) with...

Thursday, December 04, 2003 Description Rice Bombardment Procedure DAY 1 1. Filtering Rice cells with 640 兪m mesh to get small size cells. 2. Discarding the sup 3. Mountaining #4 whatman paper on the N6D 0.5 media 4. Spread 250mg cell on...

Thursday, December 04, 2003 Description PP2C assay Procedure 1. Phosphorylation of casein Casein kinaseI (NEB), 1000U/ul Sigma casein C-4765, 5% solution (56mg/ml), dilute to 10mg/ml 500ul reaction, 30oC, 4h 50 mM Tris-HCl, pH 7.5 10 mM Mg...

Thursday, December 04, 2003 Description Transient Expression Assay Using Arabidopsis Mesophyll Protoplasts : Protoplast Isolation Procedure Plant Materials: BE plants grown on the B5 medium Greenhouse-grown BE, Col, Ler and C24 plants are...