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[Cell Culture] Alkaline Lysis Plasmid Miniprep 日期：2006-07-24 14:26:22 点击：124 好评：0
Wednesday, October 01, 2003 Description REFERENCE: Molecular Cloning: A Laboratory Manual, 2nd ed., pp.1.25-1.28. Procedure Grow up a 5 ml culture overnight in the presence of the appropriate antibiotic. 2.Harvest 1.5 ml of culture by cent...
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[Cell Culture] Northern Blots 日期：2006-07-24 14:26:22 点击：139 好评：0
Tuesday, September 30, 2003 Description Send comments and updates to Dr. Bart Frank, Arthritis and Immunology Program, OMRF Horowitz, R. and Frank, M. B. Northern Blots. In: Frank, M. B. ed. Molecular Biology Protocols . (http://omrf.ouhsc...
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[Cell Culture] Phenol extraction of DNA samples 日期：2006-07-24 14:26:21 点击：157 好评：0
Monday, September 22, 2003 Description Phenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated phenol is added to an aqueous DNA sample in a microcentrifuge tube. The mixture is v...
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[Cell Culture] APOB XbaI Polymorphism 日期：2006-07-24 14:22:22 点击：35 好评：0
Saturday, May 15, 2004 Description APOB XbaI Polymorphism Procedure Xba I Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. APOBXB1: 5'-AAA TAA CCT TAA TCA TCA ATT GGT 3' 100ng/reaction Primer 2. APOBXB2: 5'-GGT TCT TAG CA...
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[Cell Culture] Phenol extraction of DNA samples ? 日期：2006-07-24 14:11:23 点击：17 好评：0
Monday, September 22, 2003 Description Phenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated phenol is added to an aqueous DNA sample in a microcentrifuge tube. The mixture is v...
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[Cell Culture] Northern Blots 日期：2006-07-24 14:11:22 点击：36 好评：0
Tuesday, September 30, 2003 Description Send comments and updates to 燚r. Bart Frank, Arthritis and Immunology Program, OMRF Horowitz, R. and Frank, M. B. Northern Blots. In: Frank, M. B. ed. Molecular Biology Protocols . (http://omrf.ouhs...
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[Cell Culture] Alkaline Lysis Plasmid Miniprep 日期：2006-07-24 14:11:22 点击：24 好评：0
Wednesday, October 01, 2003 Description REFERENCE: Molecular Cloning: A Laboratory Manual, 2nd ed., pp.1.25-1.28. Procedure Grow up a 5 ml culture overnight in the presence of the appropriate antibiotic. 2.Harvest 1.5 ml of culture by cent...
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[Cell Culture] Chromosome preparation and Fluorescence in-situ hybridizati 日期：2006-07-24 14:11:21 点击：29 好评：0
Thursday, October 16, 2003 Description Chromosome preparation and Fluorescence in-situ hybridization (FISH) Procedure 僘?To be read as micro (mu) 1.Thin slides were kept in chromic acid overnight. 2.The slides were rinsed in running tap wa...
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[Cell Culture] Transformation of Listeria monocytogenes 日期：2006-07-24 14:11:21 点击：21 好评：0
Thursday, October 16, 2003 Description A 2 days program to obtain tranformant Listeria Procedure 1 - Preparation of competent Listeria monocytogenes Day 1: 1.Prepare reagents needed: - 2X BHI (37g BHI for a final volume of 500ml, autoclave...
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[Cell Culture] H-Thymidine Uptake by Cultured Cells 日期：2006-07-24 14:11:20 点击：31 好评：0
Friday, October 17, 2003 Description H-Thymidine Uptake by Cultured Cells Procedure Grow either L cells (Mouse fibroblasts) or chick embryo fibroblasts on coverglasses and then give them H-thymidine for a short period of time. At the end o...
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