Thursday, December 04, 2003 Description ARABIDOPSIS ROOT TRANSFORMATION ASSAYS Procedure SEED STERILIZATION 1. Sterilize Arabidopsis seeds for 10 min in a solution of 50% bleach plus 0.1% SDS. 2. Rinse 5 times with sterile dH2O. 3. Place 5...

Monday, November 24, 2003 Description Electrophoretic Mobility Shift Assay (EMSA) for the detection of nuclear NF-kB Procedure Nuclear extracts For the preparation of nuclear extracts the cells are gently lysed in a NP-40 containing sucros...

Monday, November 24, 2003 Description Culture of Human Prostatic Carcinoma Cell Lines Procedure Growing and splitting the cells The human prostatic carcinoma cell lines PC3, DU145, JCA1, ALVA31, and LNCaP can be cultured in standard RPMI c...

Monday, November 24, 2003 Description Lysis in the sucrose-containing Buffer (Mito-Buffer) is supposed to prevent accidential disrupture of the mitochondria to prevent the leakage of mitochondrial proteins (such as cytochrome c) into the c...

Monday, November 24, 2003 Description Acridine orange is a fluorescent dye which will bind to the nucleic acid of bacteria and other cells. It is recommended for use for the detection of microorganisms in direct smears. It is useful for th...

Monday, November 24, 2003 Description Staining protocol Procedure Prepare cytospin slide or smear of sample. Either spray the wet preparation with Smear Fix, leave for 1-2 mins, wash off the fixative with distilled water and drain.... Or l...

Monday, November 24, 2003 Description Staining protocol Procedure For smears or cytospin preparations: Fix prepared film in methanol - 3 to 5 minutes. Stain in a mixture of 1ml of Giemsa stock plus 2mls of pH6.4 buffer and 47mls of distill...

Friday, November 21, 2003 Description Protocol for Lyophilization of bacteria Procedure Inoculate 200 ml L-broth supplemented with appropriate antibiotics with the bacteria to be lyophilized. Incubate the culture at 37C with vigorous shaki...

Friday, November 21, 2003 Description This protocol describes a simple procedure for isolating chloroplasts from spinach leaves. Procedure 1. Prepare an ice bath and pre-cool all glassware to be used (including a mortar and pestle). 2. Sel...

Friday, November 21, 2003 Description The first print of the stem will contain a lot of chlorophyll but the second or third print should reveal the position of antigens. This method transfers soluble molecules of all sorts to the membrane....