Friday, November 21, 2003 Description Thylakoid membranes are prepared Procedure Thylakoid membranes are prepared by homogenizing 40 g of fresh, deveined, market spinach (Spinacia oleracea) in 200 mL of Grinding Buffer with a chilled kitch...

Wednesday, November 19, 2003 Description Although this method was developed for use in screening clones constructed in the pRSET family of vectors (Invitrogen), it can just as easily be used with any vector system with the correct set of p...

Tuesday, November 18, 2003 Description Pheromone Halo Assay Procedure -Use sterile technique and sterile solutions throughout this method.- 1. Grow a starter culture at 30 C with shaking (250 rpm) until it reaches saturation. ***It is impo...

Tuesday, November 18, 2003 Description Yeast Prep for FACS Procedure 1. Spin down 1E7 cells in a microfuge tube for 1 minute. 2. Resuspend pellet in 1 ml of 70% EtOH. Fix for at least 60 minutes at room temperature (or up to several days a...

Thursday, November 13, 2003 Description Platelia Aspergillus (Biorad) is a 1-stage immunoenzymatic sandwich microplate kit, for the detection of antigen galactomannan Procedure In a laminar flow safety cabinet (class II), fine cut the samp...

Thursday, November 13, 2003 Description In vitro growth of seedlings Procedure sterilisation of seeds: rinse with 70% EtOH for 30 sec put in 1% bleach (sodium hypochlorite, supplemented by a few drops of Tween-20) for 5 min wash 2-4x with...

Monday, November 10, 2003 Description Protocol for determination of seed moisture content Procedure General Considerations 1. The total weight of seeds used for a moisture content determination should be sufficient to make the test accurat...

Monday, November 10, 2003 Description Plant Crosse Protocol Procedure weed out the plant pot, leave only about 3 to 8 tall and vigorous plants (in a regular 5 cm pot) on an individual inflorescence, cut off all the opened flowers (older th...

Friday, November 07, 2003 Description Cell Suspension Culture of Arabidopsis Procedure 1. Immerse Arabidopsis seeds in 10% Household Bleach for 20 min. 2. Rinse the seeds twice with 500 ml of sterile ddH2O and allow them to dry overnight i...

Friday, November 07, 2003 Description Green lab protocol for vacuum infiltration transformation of Arabidopsis Procedure 1. Sow seeds of ecotype Columbia in lightweight plastic pots prepared in the following way: mound Arabidopsis soil mix...