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[Cell Culture] COMT BglI Polymorphism 日期：2006-07-24 14:27:24 点击：304 好评：0
Saturday, May 15, 2004 Description COMT BglI Polymorphism Procedure Bgl I (modified from Chen et al., 1996) Template: Genomic DNA 100-200 ng/reaction volume of 25 l. Primer 1. COMTBGL1F 5'--TGC GGA AGG GGA CAG TGC TAC--3' 100 ng/Reaction P...
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[Cell Culture] PAH BglII Polymorphism 日期：2006-07-24 14:27:23 点击：273 好评：0
Saturday, May 15, 2004 Description PAH BglII Polymorphism Procedure BglII; LOCATED 5' OF EXON 1 Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. B2PAH1 5' -GCA GGA AAC TCT CTG ACT TTG G -3' 100 ng/reaction Primer 2. B2PAH...
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[Cell Culture] RET TaqI Polymorphism 日期：2006-07-24 14:27:23 点击：219 好评：0
Saturday, May 15, 2004 Description RET TaqI Polymorphism Procedure Taq I polymorphism located in Exon 13 Template: Genomic DNA 50-100 ng/reaction volume of 25 ul Primer 1. RETEX13F: 5'-CTC TCT GTC TGA ACT TGG GC-3' 60 ng/Reaction Primer 2....
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[Cell Culture] PAH MspI Polymorphism 日期：2006-07-24 14:27:22 点击：253 好评：0
Saturday, May 15, 2004 Description PAH MspI Polymorphism Procedure MspI; LOCATED NEAR THE 5' END OF INTRON 8 Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. PAHM 5 -GAA GCA TAT TGT ATC TGC CC -3' 100 ng/reaction Primer 2...
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[Cell Culture] HOXB3 TaqI Polymorphism 日期：2006-07-24 14:27:22 点击：226 好评：0
Saturday, May 15, 2004 Description HOXB3 TaqI Polymorphism Procedure TaqI in HOXB3; Clone pH 2-1-5 Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. H2GCK3 '5-TAT TTT TGT GAT CAA TGG AGA CC 100 ng/reaction Primer 2. H2GCK3...
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[Cell Culture] ADH2 Intron 3 RsaI Polymorphism 日期：2006-07-24 14:27:21 点击：193 好评：0
Saturday, May 15, 2004 Description ADH2 Intron 3 RsaI Polymorphism Procedure TEMPLATE: Genomic DNA 100ng/reaction volume of 25 l PRIMER 1. A2IN3DW3 5' ATA TTT ATT TTA CCC TAA ACT TAT G 3' PRIMER 2. A2IN3UP2 5' GAG CTA AAA CAT ACT TTG GAT A...
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[Cell Culture] Metaphase chromosome preparation 日期：2006-07-24 14:27:21 点击：233 好评：2
Saturday, May 15, 2004 Description Metaphase chromosome preparation Procedure Incubate culture for 72 hours in CO2 cell culture incubator, mix flask 1-2 times per day Add Colcemid (about 45 min before harvesting) Make 2 aliquots and transf...
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[Cell Culture] Protocol for RBC Lysis of Mouse Spleen 日期：2006-07-24 14:27:20 点击：270 好评：0
Monday, May 10, 2004 Description Prior to using lymphoid tissue cell suspensions for flow cytometric analysis and/or for in vitro functional assays, it is recommended to remove red blood cells. The eBioscience 1X RBC Lysis Buffer (Cat. No....
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[Cell Culture] Anti-TLR4/MD2 Neutralization of Lipid A-induced Cytokine Pro 日期：2006-07-24 14:27:20 点击：204 好评：0
Monday, May 10, 2004 Description Anti-TLR4/MD2 Neutralization of Lipid A-induced Cytokine Production by Mouse Peritoneal Cells Procedure Prepare peritoneal cells and dispense 1ml at 2x106/ml into each well of a 24-well culture plate. Pre-i...
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[Cell Culture] Anti-TLR4 Neutralization of LPS-induced Cytokine Production 日期：2006-07-24 14:27:19 点击：227 好评：0
Monday, May 10, 2004 Description Toll-like receptor 4 (TLR4) and its associated MD-2 play a role in the response of myeloid cells to LPS. While the exact mechanism is not clearly defined at the present time, antibodies to TLR4 have been re...
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