Wednesday, May 05, 2004 Description Immunofluorescence Yeast Procedure 1) Grow cells in YEPD or defined media to a concentration of 1-5 X107(protocol is for ~5 ml of exponentially growing cells). 2) Add 0.6 ml of 37% formaldehyde solution...

Wednesday, May 05, 2004 Description Yeast FACS Procedure 1. Grow cells to less than OD = 1 (i.e 2. Spin down approx 500 ul. Amount of cells should be about 5 to 10 ul volume. Too many cells does not work! 3. Resuspend in 300 l and dropwise...

Wednesday, May 05, 2004 Description These detailed instructions are for the FACScans with FACSMates, but the principles also apply to setting up 3 (or even 4) colour analysis on the FACSort. Procedure 1) It is important to set the colour c...

Wednesday, May 05, 2004 Description Neopterin [D-erythro-neopterin] belongs to the group of pteridines. Neopterin as well as other pteridines are derived in vivo from guanosinetriphophate (GTP). The enzyme GTP-cyclohydrolase-I catalyses th...

Wednesday, May 05, 2004 Description Sandwich ELISA for SCCA1 and SCCA2 Procedure 1. Dilute affinity purified rabbit anti-SCCA 1:400 in PBS (appropriate dilution of this antibody needs to be determined following each batch affinity purifica...

Wednesday, May 05, 2004 Description ELISA, Antibody capture Procedure 1. Dilute protein in PBS (2.5-5 mg/ml), add 100 ml/well. Incubate overnight at 4 C or at room temperature for 1 h. 2. Block plates with 250 ml PBS/1% BSA for overnight a...

Wednesday, May 05, 2004 Description Response to immunization with Tetanus Toxoid Vaccine is an indicator of immune function in infants and children which can be measured by comparing the amount of specific antibody detected before and afte...

Wednesday, May 05, 2004 Description IL-2 receptor alpha chain is one of the key components of the lymphoid cell receptor complex. Interleukin-2(IL-2), plays a pivotal role in initiating and potentiating the immune response. It provides a c...

Wednesday, May 05, 2004 Description This protocol is designed to increase the sensitivity of detection of HIV-1 specific CTL by the antigen-stimulation of PBMC. Stimulator cells are prepared by infecting autologous B-lymphoblastoid cell li...

Monday, May 03, 2004 Description Antibody Neutralization of IFNg-Protection from Viral Infection of L929 and A549 Cell Lines Procedure Materials L929 mouse fibroblast line (ATCC Cat. No. CCL-1) or A549 human lung carcinoma (ATCC Cat. No. C...