Thursday, December 04, 2003 Description Transient Expression Assay Using Arabidopsis Mesophyll Protoplasts : PEG Transfection Procedure All steps are carried out at 23oC Add 20 ml DNA (20-40 mg of plasmid DNA of 5 kb in size) Add 200 ml pr...

Thursday, December 04, 2003 Description Transient Expression Assay Using Maize Mesophyll Protoplasts Procedure Plant Growth Soak seeds in water for overnight. Use half vermiculite and half pelite moss and germinate seeds under light for 3...

Thursday, December 04, 2003 Description The histochemical assay to screen for expression of b-glucurodinase (gus) activity in genetically modified plants is carried out according to the method of Jefferson et al. (1987) Procedure For analy...

Thursday, December 04, 2003 Description The specific cysteine proteinase substrate Z-Phe-Arg-MCA is used as described by Barret and Kirschke (1981). The substrate, Z-Phe-Arg-NMec, which is barely fluorescent, is hydrolyzed to liberate 7-am...

Thursday, December 04, 2003 Description After extraction of soluble protein from leaves, samples are run on a SDS-PAGE gel. The method according to Sambrook et al. (1989) is used for SDS-PAGE and the method as described in the technical bo...

Friday, December 19, 2003 Description This assay uses the zigmond chamber to study chemotaxis of spermatozoa or other migratory cells. The chamber consists of two wells separated by a bridge. The culture medium is placed on the bridge, cov...

Saturday, January 17, 2004 Description In vitro whole cell assay by [H3] Hypoxanthine uptake assay Procedure [H3] Hypoxanthine uptake assay (with respect to red blood cell cultures of P. falciparum Dispense 25 ul complete RPMI in different...

Thursday, January 29, 2004 Description This protocol is designed to synchronize HeLa cells at the G1/S border (actually all G1). It is highly effect and gives almost complete synchronization in the culture. Note that this protocol will not...

Tuesday, March 16, 2004 Description Wright's stain is a Romanowsky type metachromatic stain made by mixing old or specially treated methylene blue dye with eosin in a methanol diluent. Basic components of the cell, such as hemoglobin or ce...

Tuesday, March 16, 2004 Description When one refers to particle counting in the clinical laboratory, he or she is usually referring to the counting of blood cells. Cells are, in fact, particles, and these particles are suspended in a liqui...