Tuesday, March 16, 2004 Description S閦ary cells are peripheralized malignant T cells seen in some patients with mycosis fungoides. Peripheral blood smears are stained with Wright-Giemsa stain and evaluated by the director of the laborator...

Tuesday, March 16, 2004 Description This procedure describes the freezing of the rodent cells with serum. Procedure Filter sterilize 700ml of RPMI-1640 (2mM L-Glutamine) and 200ml fetal bovine serum (FBS) through a 0.22祄 filter. Add 100ml...

Monday, May 03, 2004 Description Culture of Dissociated Sympathetic Neurons Procedure 1. Use day 9 chicken embryos. 2. Dissect sympathetic chains in Ham's F12 media. 3. Dissociate ganglia through incubation with 0.25% trypsin for 20 min at...

Monday, May 03, 2004 Description Culture of Dissociated Cortical Neurons Procedure 1. Use 7 to 8 day chicken embryos 2. Dissect forebrain in Ham's F12 media, peel off all meninges and then separate cortex from basal ganglia 3. Dissociate n...

Monday, May 03, 2004 Description Conditioned Medium for Retinal Explant Culture Procedure Remove E14.5 rat retinae with lens intact in prewarmed explant culture medium. Immediately place 4 retinae on a polycarbonate filter (1 micron pore s...

Monday, May 03, 2004 Description Organotypic Culture Procedure Organotypic Hippocampal Slice Cultures 1. MED probe coating: Polyethylenimine (PEI) method a. Sterilize the MED probe with 70% ethanol (DO NOT autoclave). b. Dry up the MED pro...

Monday, May 03, 2004 Description Preparation of a dispersed neuron culture of rat suprachiasmatic nucleus (SCN) Procedure Rat pups were anesthetized by hypothermia and decapitated. Frontal sections of the hypothalamus (700 祄 thick) were o...

Monday, May 03, 2004 Description Weskamp, G., and Otten, U. (1987). An enzyme-linked immunoassay for nerve growth factor (NGF): A tool for studying regulatory mechanisms involved in ngf production in brain and in peripheral tissues. Journa...

Monday, May 03, 2004 Description Dissociated Retina Immunohistochemistry Procedure before starting see criteria/guidelines for dissociated cell scoring ?Wash the tissue (freshly dissected or explant) in prewarmed PBS (6 ml in a 6 cm dish)....

Monday, May 03, 2004 Description Some other useful protocols can be found in the following references: Caltuvetti et. al., 1993, J. Pathology 171:83 and Shi et. al., 1991 J. Hist. Cytochem. 39:741. Procedure Place the slides in a plastic c...