生物技术 / 分子生物学

分子生物学 Beta-Lactamase Induction (Crude Preparation)

Wednesday October 01 2003 Description REFERENCE Gootz Sanders. A.A.C. Procedure 1. Inoculate regular 关键词：Beta、Lactamase...

2005-07-18 00:00:00 285 0

Sunday October 19 2003 Description Gel Mobility Shift Assay Using Magnesium Chloride and EDTA Proced...

2005-07-18 00:00:00 342 0

Sunday October 19 2003 Description This protocol describes a competitive ligand binding assay for co 关键词：Competitive、Ligand...

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Monday November 10 2003 Description Inverse PCR ProtocolThis method can be used directly for pBIN19...

2005-07-18 00:00:00 435 0

Monday November 10 2003 Description Leaf GUS Assay Procedure This protocol is for small samples usua...

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Tuesday November 18 2003 Description Yeast Colony PCR Procedure PCR mixtureCombine reaction mix on i...

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Tuesday November 18 2003 Description UV Mutagenesis Procedure 1 Grow cells 2-3 days to stationary ph...

2005-07-18 00:00:00 341 0

Tuesday November 18 2003 Description Disruption by Fusion PCR Procedure 1 In separate PCR reactions...

2005-07-18 00:00:00 300 0

本文详细介绍了氯化钙法制备大肠杆菌感受态细胞及转化的标准实验方案。包括菌液培养至OD600=0.6、CaCl2处理、热激转化等关键步骤，并提供了试剂配方和操作注意事项。该方案适用于分子生物学中质粒DNA的导入，是基因克隆和表达研究的基础技术。...

2005-07-18 00:00:00 636 0

本文介绍了一种简易制备大肠杆菌感受态细胞的方法，该细胞可在4摄氏度下保存一周。详细描述了从过夜培养、对数生长期培养、冰浴、离心、重悬到最终获得感受态细胞的全过程，并提供了转化方案和KMES缓冲液配方。该方法适用于实验室常规转化操作，具有操作简便、保存时间长的优点。...

2005-07-18 00:00:00 321 0

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